茶园阴坡和阳坡土壤微生物群落结构与土壤理化因子的关系  

Relationship between soil microbial community structure and soil physicochemical factors of shady slope and sunny slope of tea garden

作　　者：刘威 王晶晶 师梦楠 张文静 王子浩 郭桂义 张永瑞 LIU Wei;WANG Jing-jing;SHI Meng-nan;ZHANG Wen-jing;WANG Zi-hao;GUO Gui-yi;ZHANG Yong-rui(College of Tea Science,Xinyang Agriculture and Forestry University,Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan,Xinyang 464000,China;Henan Dabieshan National Field Observation and Research Station of Forest Ecosystem,Zhengzhou 450046,China;Xinyang Academy of Ecological Research,Xinyang 464000,China)

机构地区：[1]信阳农林学院茶学院/河南省豫南茶树资源综合开发重点实验室,河南信阳464000 [2]河南大别山森林生态系统国家野外科学观测研究站,河南郑州450046 [3]信阳生态研究院,河南信阳464000

出　　处：《江苏农业学报》2024年第2期251-259,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2021YFD1601103);河南省科技攻关项目(232102110212);信阳生态研究院开放基金项目(2023XYQN23)。

摘　　要：采用Illumina MiSeq高通量测序技术探究茶园阴坡和阳坡土壤细菌、真菌群落结构特点,并开展土壤理化因子对土壤细菌、真菌群落结构的影响分析。结果表明,茶园阴坡土壤细菌和真菌分类操作单元数量均高于阳坡。在门分类水平上,阴坡土壤中相对丰度最高的细菌门为酸杆菌门,阳坡为变形菌门,而阴坡和阳坡土壤中相对丰度最高的真菌门均为子囊菌门,相对丰度均超过55.00%;在目分类水平上,茶园阴坡和阳坡土壤相对丰度最高的细菌、真菌均不同。物种多样性分析结果显示,阴坡和阳坡茶园土壤间细菌和真菌物种多样性和物种相对丰度均无显著差异。阴坡和阳坡富集的细菌不同,而富集的真菌较为相似。冗余分析结果显示,细菌受土壤因子的影响大于真菌,且土壤速效磷含量是影响土壤细菌和真菌群落最主要的环境因子。Illumina MiSeq high-throughput sequencing technology was used to explore the bacterial and fungal community structure characteristics of the shady slope and sunny slope of tea garden,and analysis of the effects of soil physical and chemical factors on the bacterial and fungal community structures was conducted.The results showed that the number of bacterial and fungal operational taxonomic units(OTUs)in the shady slope soil was higher than that in the sunny slope soil.At the phylum classification level,the bacterial phyla in the shady slope soil and the sunny slope soil with the highest relative abundance were Acidobacteria and Proteobacteria respectively,and the most abundant fungal phylum in both slopes was Ascomycota,with a relative abundance exceeding 55.00%.At the order classification level,the most abundant bacteria and fungi in the shady slope soil and sunny slope soil were different.Species diversity analysis showed that there was no significant difference in species diversity or richness of bacterial and fungal communities in the soils between the shady and sunny slopes of the tea garden.Different bacterial species were enriched in the shady slope and sunny slope soils,while the fungal species enriched were similar.Redundancy analysis found that bacteria received greater influence by soil factors than fungi,and soil available phosphorus content was the most important environmental factor affecting soil bacterial and fungal communities.

关 键 词：茶园 阴坡和阳坡 土壤微生物 群落结构 土壤理化因子 

分 类 号：S154.3[农业科学—土壤学]