饲料中添加高羊毛氨酸硒对陕北白绒山羊羯羊瘤胃古菌结构与组成的影响  

Effects of dietary supplementation with selenohomolanthionine on the structure and composition of rumen archaea in Shaanbei white cashmere wether goats

作　　者：李托 李陇平[1] 屈雷[1] LI Tuo;LI Long-ping;QU Lei(Shaanxi Province Engineering&Technology Research Center of Cashmere Goat,Yulin University,Yulin 719000,China;College of Life Sciences,Yulin University,Yulin 719000,China)

机构地区：[1]榆林学院陕西省陕北绒山羊工程技术研究中心,陕西榆林719000 [2]榆林学院生命科学学院,陕西榆林719000

出　　处：《江苏农业学报》2024年第2期331-341,共11页Jiangsu Journal of Agricultural Sciences

基　　金：榆林市科技局产学研项目(CXY-2022-54);陕西省自然科学基础研究计划项目(2022JQ-187)。

摘　　要：为明确不同剂量高羊毛氨酸硒(SeHLan)对陕北白绒山羊羯羊瘤胃古菌结构与组成的影响,本研究选取发育一致的陕北白绒山羊羯羊32只,均分为4组,分别饲喂硒含量为0.016 mg/kg(CK)、0.300 mg/kg、0.600 mg/kg和1.200 mg/kg的饲料,饲养70 d后,采集羯羊瘤胃液,提取DNA并利用古菌特异性引物进行PCR扩增,对古菌V4-V5区进行高通量测序和羯羊瘤胃液古菌结构与组成分析。结果表明:随着SeHLan添加量增加,各处理瘤胃液古菌α多样性指数呈现出先降低后增高的变化趋势,但处理间瘤胃液古菌α多样性指数差异均不显著。各处理瘤胃液,在门水平上广古菌门(Euryarchaeota)古菌,在科水平上甲烷短杆菌科(Methanobacteriaceae)古菌,在属水平上甲烷短杆菌属(Methanobrevibacter)古菌的相对丰度均接近80%,且随着饲料中SeHLan添加量增加,相对丰度呈现先降低后升高的变化趋势。0.600 mg/kg硒含量饲料处理的羯羊瘤胃液在门水平上广古菌门(Euryarchaeota)古菌,在科水平上甲烷杆菌科(Methanobacteriaceae)古菌,在属水平上甲烷短杆菌属(Methanobrevibacter)古菌,相对丰度显著低于其他3个处理。综上所述,饲料中添加SeHLan显著影响陕北白绒山羊羯羊瘤胃液古菌组成及结构,0.600 mg/kg硒含量饲料处理的羯羊瘤胃产甲烷菌丰度显著低于其他处理。In order to clarify the effects of different doses of selenohomolanthionine(SeHLan)on the structure and composition of rumen archaea in Shaanbei white cashmere wether goats,thirty-two Shaanbei white cashmere wether goats with consistent development were selected and divided into four groups randomly.They were fed diets with selenium contents of 0.016 mg/kg(CK),0.300 mg/kg,0.600 mg/kg and 1.200 mg/kg,respectively.After the 70-day feeding experiment,the rumen fluid of all the experimental wether goats was collected,DNA was extracted,and PCR amplification was performed using archaeal-specific primers.The archaeal V4-V5 region was subjected to high-throughput sequencing analysis,and the structure and composition of archaea in the rumen fluid of wethers were analyzed.The results showed that with the increase of SeHLan addition,theαdiversity index of rumen fluid archaea showed a trend of decreasing first and then increasing,but the difference ofαdiversity index of rumen fluid archaea between treatments was not significant.The relative abundance of Euryarchaeota archaea at the phylum level,Methanobacteriaceae archaea at the family level and Methanobrevibacter archaea at the genus level in rumen fluid of each treatment was close to 80%,and with the increase of dietary SeHLan addition,the relative abundance showed a trend of decreasing first and then increasing.The relative abundance of Euryarchaeota archaea at the phylum level,Methanobacteriaceae archaea at the family level and Methanobrevibacter archaea at the genus level in rumen fluid of wether goats treated with 0.600 mg/kg selenium content diet was significantly lower than that of the other three treatments.In summary,the addition of SeHLan to the diet significantly affected the composition and structure of rumen fluid archaea in Shanbei white cashmere goats.The abundance of rumen methanogens in wethers treated with 0.600 mg/kg selenium content diet was significantly lower than that in other treatments.

关 键 词：高羊毛氨酸硒 陕北白绒山羊羯羊 高通量测序 瘤胃 古菌 

分 类 号：S827.8[农业科学—畜牧学]