基于全长转录组的蚕豆WRKY基因家族分析及耐盐胁迫相关候选基因挖掘  

Analysis of WRKY gene family based on full-length transcriptome and mining of salt stress candidate genes in Vicia faba

作　　者：周恩强 周瑶 姚梦楠 王学军[1] 赵娜[1] 缪亚梅[1] 王永强[1] 薛冬 李波[1] 汪凯华[1] 顾春燕[1] 魏利斌 ZHOU En-qiang;ZHOU Yao;YAO Meng-nan;WANG Xue-jun;ZHAO Na;MIAO Ya-mei;WANG Yong-qiang;XUE Dong;LI Bo;WANG Kai-hua;GU Chun-yan;WEI Li-bin(Jiangsu Yanjiang Institute of Agricultural Sciences,Nantong 226012,China)

机构地区：[1]江苏沿江地区农业科学研究所,江苏南通226012

出　　处：《江苏农业学报》2024年第1期14-30,共17页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省种业振兴揭榜挂帅项目[JBGS(2021)056];江苏省农业科技自主创新基金项目[CX(22)2011];国家食用豆产业技术体系项目(CARS-08-Z10);江苏沿江地区农业科学研究所青年科技基金项目[YJ(2022)002];江苏沿江地区农业科学研究所博士基金项目[YJBS(2021)001]。

摘　　要：WRKY基因是植物特有的转录因子基因,能够调控植物的生长发育和胁迫响应。为了鉴定蚕豆WRKY基因家族成员,揭示其进化关系并挖掘与盐胁迫相关的候选WRKY基因,本研究在完成蚕豆全长转录组测序(9个样品)和二代转录组测序(27个样品)的基础上,利用生物信息学方法对WRKY转录因子基因进行鉴定与分析,并通过拟南芥同源基因比对挖掘盐胁迫相关的候选VfWRKY基因。结果表明,蚕豆全长转录组测序共获得53.84 Gb数据量,通过比对和校正最终获得58885条转录本序列信息;基于蚕豆全长转录组共鉴定出113个WRKY家族成员,氨基酸数目为153~737 aa,等电点为4.84~9.87,113个WRKY家族蛋白质全部定位于细胞核中;根据拟南芥WRKY家族系统发育特征,VfWRKY基因家族可分为3组,分别为group 1(38个VfWRKY)、group 2(61个VfWRKY)、group 3(14个VfWRKY);Motif 1和Motif 3是VfWRKY基因家族的特征基序,并对应WRKY保守结构域,在进化过程中较为保守;VfWRKY基因家族主要富集在植物MAPK信号通路、植物与病原菌相互作用和剪接体3个通路中;通过同源比对,在蚕豆中发现14个盐胁迫相关候选WRKY基因,且主要在根中高表达。该研究结果为蚕豆的遗传学研究提供了丰富的参考数据,也为创制耐盐蚕豆新品种提供了基因信息。The WRKY gene is a plant-specific transcription factor gene that regulates plant growth and development and stress response.In order to identify the WRKY gene family members of Vicia faba,reveal their evolutionary relationship and mine candidate WRKY genes related to salt stress,in this study,the WRKY transcription factor genes were identified and analyzed by bioinformatics methods based on the full-length transcriptome(nine samples)and second-generation transcriptome sequencing(27 samples)of Vicia faba,and the candidate VfWRKY genes related to salt stress were excavated by homologous gene alignment of Arabidopsis thaliana.The results showed that 53.84 Gb data were obtained from the full-length transcriptome sequencing of Vicia faba,and 58885 transcript sequence information was finally obtained through alignment and correction.A total of 113 WRKY family members were identified based on the full-length transcriptome of Vicia faba,with amino acid numbers of 153-737 aa and isoelectric points of 4.84-9.87,all 113 WRKY family proteins were localized in the nucleus.According to the phylogenetic characteristics of Arabidopsis WRKY family,the VfWRKY gene family was divided into three groups:group 1(38 VfWRKY),group 2(61 VfWRKY),and group 3(14 VfWRKY).Motif 1 and Motif 3 were characteristic motifs of the VfWRKY gene family and corresponded to the WRKY domain,which were relatively conserved during evolution.The VfWRKY gene family was mainly enriched in three pathways:plant-MAPK signaling pathway,plant-pathogen interaction pathway and spliceosome pathway.Fourteen candidate WRKY genes related to salt stress were found in Vicia faba by homology alignment,and they were mainly highly expressed in the roots.The results of this study can provide rich reference data for the genetics research of Vicia faba,and also provide gene information for creating new varieties of salt-tolerant Vicia faba.

关 键 词：WRKY基因 蚕豆 全长转录组 盐胁迫 进化分析 

分 类 号：S551.4[农业科学—作物学]