麻风树JcHDZ28基因克隆与功能分析  

Cloning and function analysis of JcHDZ28 gene from Jatropha curcas L.

作　　者：唐跃辉 赵雨凡 蒋心言 张英颖 王寒 包欣欣 范雨杰 李彤 TANG Yue-hui;ZHAO Yu-fan;JIANG Xin-yan;ZHANG Ying-ying;WANG Han;BAO Xin-xin;FAN Yu-jie;LI Tong(School of Life Sciences and Agronomy,Zhoukou Normal University,Zhoukou 466001,China)

机构地区：[1]周口师范学院生命科学与农学学院,河南周口466001

出　　处：《江苏农业学报》2024年第1期39-46,共8页Jiangsu Journal of Agricultural Sciences

基　　金：河南省高等学校重点科研项目(24A180029);河南省周口师范学院大学生创新创业训练计划项目(202210478038、202210478040)。

摘　　要：HD-Zip家族基因与植物的生长和对环境胁迫的抗性密切相关,被认为是作物改良的关键因子。在本研究中,我们通过RT-PCR技术从麻风树中克隆了1个HD-Zip家族基因,命名为JcHDZ28。JcHDZ28基因包含1个882 bp的开放阅读框,编码1个含有293个氨基酸的蛋白质。氨基酸序列分析结果表明,JcHDZ28含有高度保守的同源结构域和亮氨酸拉链(LZ)基序。表达模式分析结果表明,JcHDZ28基因在种子中的相对表达量最高,且盐胁迫下调该基因的表达。亚细胞定位分析结果表明,JcHDZ28基因编码1个核定位蛋白。过表达JcHDZ28基因增加了转基因拟南芥对盐胁迫的敏感性,且在盐胁迫条件下,转JcHDZ28基因拟南芥叶片脯氨酸含量显著低于野生型拟南芥,相对电导率显著高于野生型拟南芥,非生物胁迫相关基因在转JcHDZ28基因拟南芥中的表达也显著低于在野生型拟南芥中的表达。本研究结果可以为进一步阐明HD-Zip转录因子基因JcHDZ28在麻风树生长发育和响应非生物胁迫中的功能提供理论依据。The genes of HD-Zip family are closely related to plant growth and resistance to environmental stress,and are considered to be key factors in crop improvement.In the study,we cloned a HD-Zip family gene from Jatropha curcas L.using RT-PCR technology,and named JcHDZ28.The JcHDZ28 gene contained an open reading frame of 882 bp,and encoded a protein with 293 amino acids.Amino acid sequence analysis showed that JcHDZ28 contained a highly conserved homologus domain and leucine zipper(LZ)motif.Expression profile analysis showed that the relative expression of JcHDZ28 gene was the highest in seeds,and salt stress inhibited the expression of this gene.Subcellular localization analysis showed that JcHDZ28 gene encoded a nuclear localization protein.Overexpression of JcHDZ28 gene increased the sensitivity of transgenic Arabidopsis to salt stress.Under salt stress,the proline content of transgenic plants was significantly lower than that of wild type,and the relative conductivity was significantly higher than that of wild type.The expression of abiotic stress-related genes in JcHDZ28 transgenic plants was also significantly lower than that in wild type.The results of this study can provide a theoretical basis for further elucidating the function of HD-Zip transcription factor gene JcHDZ28 in the growth,development and response to abiotic stress in Jatropha curcas L.

关 键 词：麻风树 JcHDZ28 HD-ZIP 盐胁迫 转基因拟南芥 

分 类 号：S511[农业科学—作物学]