结球甘蓝CBF家族特征分析及低温诱导基因BoCBF1、BoCBF2a和BoCBF3表达分析  

Characterization of CBF family in cabbage and the expression analysis of low-temperature-induced genes BoCBF1,BoCBF2a,and BoCBF3

作　　者：张伟[1] 余方伟 李建斌[1] 于利[1] 王神云[1] ZHANG Wei;YU Fang-wei;LI Jian-bin;YU Li;WANG Shen-yun(Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014

出　　处：《江苏农业学报》2024年第1期156-164,共9页Jiangsu Journal of Agricultural Sciences

基　　金：南京市科技计划项目(202109021);江苏省重点研发计划项目(BE2021376);江苏省种业振兴揭榜挂帅项目[JBGS(2021)067];国家自然科学基金项目(31902009);国家现代农业产业技术体系建设专项(CARS-23-G42)。

摘　　要：转录因子CBF在植物对低温胁迫的响应和增强植物的抗寒性方面发挥着重要作用。为分析结球甘蓝CBF家族成员的氨基酸序列特征,探究其是否受低温诱导表达,本研究利用结球甘蓝923全基因组数据库对其进行蛋白质理化特征、系统发育关系、编码基因结构以及2℃低温胁迫下编码基因表达水平分析。结果表明,共鉴定到7个BoCBF基因,系统发育分析后分成2个亚组(Ⅰ和Ⅱ)。BoCBF蛋白的氨基酸长度为203~283 aa,全部是亲水性蛋白质。在结球甘蓝02-12中,BoCBF1、BoCBF2a和BoCBF3基因在叶、花、芽和角果中基本不表达,在愈伤组织、根和茎中表达量较低。转录组测序和实时荧光定量PCR分析发现,在耐寒结球甘蓝923和不耐寒结球甘蓝D9中,BoCBF2b、BoCBF2c基因不受低温诱导表达,BoCBF2a基因低温诱导表达最为迅速,其次是BoCBF1和BoCBF3基因。BoCBF1、BoCBF2a和BoCBF3基因的相对表达量在低温胁迫3~6 h达到最大值,相对表达量达到最大值后急剧下降,在24 h降至最低。本研究结果为后续开展BoCBF1、BoCBF2a和BoCBF3基因调控结球甘蓝响应低温胁迫机理研究奠定了基础。Transcription factor CBF plays an important role in response to low temperature stress and enhancing cold resistance in plants.In order to analyze the amino acid sequence characteristics of CBF family members in cabbage and explore whether the expression of BoCBF genes was induced by low temperature,the whole genome database of cabbage 923 was used to identify BoCBF gene members and analyze the protein physicochemical properties,phylogenetic relationships,gene structures,and gene expression levels under 2℃low temperature stress.The results showed that a total of seven BoCBF genes were identified and divided into two subgroups(ⅠandⅡ)after phylogenetic analysis.The amino acid length of BoCBF proteins ranged from 203 aa to 283 aa,all of which were hydrophilic proteins.BoCBF1,BoCBF2a,and BoCBF3 genes were not expressed in leaves,flowers,buds and siliques of cabbage 02-12,and the expression levels were low in callus,roots and stems of cabbage 02-12.Transcriptome sequencing and real-time fluorescence quantitative PCR analysis revealed that the expression of BoCBF2b and BoCBF2c genes was not induced by low temperature in cold-intolerant cabbage 923 and cold-intolerant cabbage D9,while BoCBF2a gene was most rapidly induced by low temperature,followed by BoCBF1 and BoCBF3 genes.The relative expression levels of BoCBF1,BoCBF2a,and BoCBF3 genes reached the maximum at 3-6 h after low temperature stress and decreased sharply after reaching the maximum,and reached the lowest at 24 h.The results of the present study provide a basis for the subsequent study on the mechanism of BoCBF1,BoCBF2a and BoCBF3 genes in response to low temperature stress in cabbage.

关 键 词：结球甘蓝 CBF基因家族 低温胁迫 基因表达 

分 类 号：S635[农业科学—蔬菜学]