缺血性疾病患者血清lncRNA PVT1和FOXM1表达水平及联合心脏磁共振延迟强化成像与预后的关系  

The Expression Levels of lncRNA PVT1 and FOXM1 in Serum of Patients with Ischemic Diseases and the Relationship between the Expression Levels Combined Late Gadolinium Enhancement Magnetic Resonance Imaging and Prognosis

作　　者：尹晓翔[1] 赵森[1] 郭颖 刘梦雯 庄琰 YIN Xiao-xiang;ZHAO Sen;GUO Ying;LIU Meng-wen;ZHUANG Yan(Department of Medical Imaging,The First Affiliated Hospital of Henan University,Kaifeng 475000,Henan Province,China;Department of Medical Administration,The First Affiliated Hospital of Henan University,Kaifeng 475000,Henan Province,China)

机构地区：[1]河南大学第一附属医院医学影像科,河南开封475000 [2]河南大学第一附属医院医政科,河南开封475000

出　　处：《中国CT和MRI杂志》2024年第3期73-75,共3页Chinese Journal of CT and MRI

基　　金：河南省医学科技攻关计划项目(LHGJ20210565)。

摘　　要：目的 探讨缺血性疾病患者血清长链非编码RNA浆细胞瘤转化迁移基因1(lncRNA PVT1)和叉头框转录因子M1(FOX M1)表达水平及联合心脏钆对比剂延迟增强磁共振成像(LGE-MRI)与预后的关系。方法 选取2021年2月-2022年2月我院收治的缺血性心脏病患者118例即为研究组,随访一年根据随访过程中是否发生主要心脏不良事件(MACE),分为MACE组32例,无MACE组86例。同期选择在我院体检健康的志愿者118例为对照组。实时荧光定量PCR(qRT-PCR)检测血清lncRNA PVT1的相对表达量。采用酶联免疫吸附试验(E LISA)检测FOXM1水平。受试者工作特征(ROC)曲线分析LGE-MRI、血清lncRNA PVT1和FOXM1对缺血性心脏病患者预后发生MACE的预测价值。结果 研究组患者DBP、SBP、TG、TC、 LDL-C、GLU水平与对照组相比显著升高,HDL-C水平显著降低(P<0.05)。与对照组相比,研究组的血清中lncRNA PVT1和FOXM1水平显著升高(P<0.05)。与无MACE组相比,MACE组患者DBP、SBP、TC、LDL-C水平显著升高, HDL-C水平显著降低(P<0.05)。与无MACE组相比,MACE组患者血清中lncRNA PVT1和FOXM1水平显著升高(P<0.05)。MACE组的LGE-MRI阳性数量显著高于无MACE组(P<0.)05。与LGE-MRI、血清lncRNA PVT1和FOXM1单独预测相比,三者联合预测MACE发生的AUC更高(Z=7.221,P<0.001;Z=7.737,P<0.001;Z=7.091,P<0.001)。结论 缺血性心脏病预后发生MACE的患者血清lncRNA PVT1和FOXM1水平呈高表达,二者联合LGE-MRI对MACE的发生有一定的预测价值。Objective To investigate the expression levels of long non-coding RNA plasmacytoma variant translocation gene 1(lncRNA PVT1)and fork head box transcription factor M1(FOXM1)in serum of patients with ischemic disease,and the relationship between the expression levels combined with late gadolinium enhancement magnetic resonance imaging(LGE-MRI)and prognosis.MethodsFrom February 2021 to February 2022,118 patients with ischemic heart disease admitted to our hospital were regarded as the study group,during the one-year follow-up,32 patients were grouped into the MACE group and 86 patients without MACE group based on whether major adverse cardiac events(MACE)occurred during the follow-up process.During the same period,118 healthy volunteers who underwent physical examinations in our hospital were regarded as the control group.Real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect the relative expression of lncRNA PVT1 in serum.Enzyme linked immunosorbent assay(ELISA)was applied to detect FOXM1 level.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of LGE-MRI,serum lncRNA PVT1,and FOXM1 for MACE in patients with ischemic heart disease.ResultsThe levels of DBP,SBP,TG,TC,LDL-C,and GLU in the study group were obviously higher than those in the control group,while the level of HDL-C was obviously lower(P<0.05).Compared with the control group,the serum levels of lncRNA PVT1 and FOXM1 in the study group were obviously increased(P<0.05).Compared with the non MACE group,the levels of DBP,SBP,TC,and LDL-C in the MACE group were obviously higher,while the level of HDL-C was obviously lower(P<0.05).Compared with the non MACE group,the serum levels of lncRNA PVT1 and FOXM1 in the MACE group were obviously increased(P<0.05).The number of LGE-MRI positive in the MACE group was obviously higher than that in the non MACE group(P<0.05).Compared with LGE-MRI,serum lncRNA PVT1,and FOXM1 alone,the combination of the three in predicting MACE had a higher AUC(Z=7.221,P<0.001;Z=7.737,P<0.00

关 键 词：长链非编码RNA浆细胞瘤转化迁移基因1 叉头框转录因子M1 心脏钆对比剂延迟增强磁共振成像 预后 缺血性疾病 

分 类 号：R445.2[医药卫生—影像医学与核医学]