低压氧舱处置时间对高海拔大鼠心搏骤停模型制备的影响  

Discussion on the relationship between the disposal time of hypobaric oxygen chamber and the establishment of rat cardiac arrest model at high altitude

作　　者：刘洁 马增文 石晓旭 吴妍 南措吉[1] 宋凤卿[2] 张斌[1] Liu Jie;Ma Zengwen;Shi Xiaoxu;Wu Yan;Nan Cuoji;Song Fengqing;Zhang Bin(Department of Emergency,People's Hospital Affiliated to Qinghai University(Qinghai Provincial People's Hospital),Plateau Branch,Institute of Cardiopulmonary and Cerebral Resuscitation,Sun Yat-sen University,Xining 810000,Qinghai,China;Department of Emergency,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Cardiopulmonary and Cerebral Resuscitation Institute,Sun Yat-sen University,Guangzhou 510006,Guangdong,China)

机构地区：[1]青海大学附属人民医院(青海省人民医院)急诊科,中山大学心肺脑复苏研究所高原分所,西宁810000 [2]中山大学孙逸仙纪念医院急诊科,中山大学心肺脑复苏研究所,广东广州510006

出　　处：《中华危重病急救医学》2024年第1期82-85,共4页Chinese Critical Care Medicine

基　　金：青海省科技计划项目(2020-ZJ-771)。

摘　　要：目的建立高海拔低压低氧环境大鼠心搏骤停模型,探讨低压氧舱处置时间对建立高海拔大鼠心搏骤停模型的影响。方法以SPF级健康雄性SD大鼠作为研究对象,实验分别在2个不同海拔地区开展。将中山大学心肺脑复苏研究所高原分所(青海西宁)的实验大鼠称重、编号,放入低压氧舱中饲养(模拟海拔高度3000 m,升降速15 m/min,温度20℃,舱内压力69.5 kPa,舱内氧气压力14.5 kPa),饲养30 d后按照随机数字表法选取30只大鼠,采用窒息法制备心搏骤停模型,作为低压低氧30 d组;饲养60 d后再次随机选取40只大鼠制备心搏骤停模型,作为低压低氧60 d组。中山大学心肺脑复苏研究所(广东广州)采用相同方法随机选取30只大鼠制备心搏骤停模型,作为平原对照组。比较各组大鼠制模前体质量及制模过程中窒息诱导时间的差异。结果最终低压低氧30 d组有16只大鼠完成心搏骤停模型制备,低压低氧60 d组有22只大鼠完成心搏骤停模型制备。平原对照组、低压低氧30 d组与低压低氧60 d组大鼠制模前体质量差异无统计学意义〔g:429.00(389.25,440.75)、440.00(415.50,486.25)、440.00(400.00,452.50),均P>0.05〕。低压低氧60 d组大鼠窒息诱导时间较低压低氧30 d组明显延长(s:294.59±75.39比234.31±93.86,P<0.01),甚至约为平原对照组的1.4倍(s:294.59±75.39比208.73±30.88,P<0.01);而低压低氧30 d组大鼠窒息诱导时间与平原对照组差异无统计学意义(P>0.05)。结论低压氧舱处置60 d的大鼠更适用于制备高海拔心搏骤停模型,也符合高海拔大鼠氧储备和耐缺氧的机体特点。Objective To establish the rat cardiac arrest model in high-altitude hypobaric hypoxia environment,and to explore the effect of the treatment time in the hypobaric oxygen chamber on the reproduction of high-altitude rat cardiac arrest model.Methods SPF grade healthy male Sprague-Dawley(SD)rats were used as observation subjects.The experiment was conducted in two different altitude areas.The rats from the Plateau Branch of Institute of Cardiopulmonary and Cerebral Resuscitation of Sun Yat-sen University(Xining,Qinghai)were weighed and numbered,and they were placed in a hypobaric oxygen chamber(simulated altitude of 3000 meters,speed of ascent and descent of 15 m/min,temperature of 20℃,cabin pressure of 69.5 kPa,cabin oxygen pressure of 14.5 kPa).After 30 days of feeding,the rats were obtained according to random number table method,and the cardiac arrest model was established by asphyxia method as the 30-day hypobaric hypoxia group.After 60 days of feeding,rats were randomly selected again,and the cardiac arrest model was established as the 60-day hypobaric hypoxia group.Thirty rats were randomly selected from the Institute of Cardiopulmonary Cerebral Resuscitation at Sun Yat-sen University(Guangzhou,Guangdong)by the same method,and the cardiac arrest model was established as the plain control group.The differences in the body weight of rat modeling precursors and the induction time of asphyxia during the modeling process among different groups were compared.Results Finally,cardiac arrest model was established in 16 rats in the 30-day hypobaric hypoxia group and in 22 rats in the 60-day hypobaric hypoxia group.There was no significant difference in the body weight of rats before modeling among the plain control group,30-day hypobaric hypoxia group and 60-day hypobaric hypoxia group[g:429.00(389.25,440.75),440.00(415.50,486.25),440.00(400.00,452.50),all P>0.05].The asphyxia induction time of rats in the 60-day hypobaric hypoxia group was significantly longer than that in the 30-day hypobaric hypoxia group(s:294.5

关 键 词：高海拔地区 心搏骤停 大鼠模型 低压氧舱 

分 类 号：R541.78[医药卫生—心血管疾病] R-332[医药卫生—内科学]