基于^(13)C同位素标记法探究连作对丹参生长及光合碳分配的影响  

Effects of Continuous Cropping on Growth and Photosynthetic Carbon Distribution of Salvia miltiorrhiza Based on ^(13)C Isotope Labeling

作　　者：孟缘 付心雨 鞠吉东 周冰谦 卢恒 王晓 郭兰萍[3] 刘伟[2] Meng Yuan;Fu Xinyu;Ju Jidong;Zhou Bingqian;Lu Heng;Wang Xiao;Guo Lanping;Liu Wei(College of Parmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Qilu University of Technology(Shandong Academy of Sciences)/Shandong Analysis and Testing Center,Jinan 250014,China;Chinese Medicine Resource Center,Chinese Academy of Traditional Chinese Medicine,Beijing 100700,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]齐鲁工业大学(山东省科学院)/山东省分析测试中心,山东济南250014 [3]中国中医科学院中药资源中心,北京100700

出　　处：《山东农业科学》2024年第2期95-103,共9页Shandong Agricultural Sciences

基　　金：国家自然科学基金面上项目(82173917);国家现代农业产业技术体系项目(CARS-21);中央本级重大增减支项目(2060302);山东省重点研发计划项目(2021ZDSYS12);齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2022PX093)。

摘　　要：以1年龄盆栽丹参为研究对象,设置^(13)C脉冲标记处理与^(12)C正常处理,应用^(13)C脉冲标记法研究连作与非连作丹参光合碳分配规律,比较植株标记40 d后的形态学与理化指标差异,分析丹参地上部、根部以及根际土壤碳的^(13)C丰度、碳同位素比率、^(13)C原子百分比以及单位干重样品的^(13)C总量,以明确连作对丹参生长与光合作用的影响机理。结果表明,连作条件下,丹参各部分生物量与叶绿素含量明显下降,抗氧化酶活性升高;有效成分中的丹参酮Ⅰ、丹参酮Ⅱ_(A)、二氢丹参酮Ⅰ以及迷迭香酸含量均降低;连作显著影响^(13)C-光合碳分配比例,非连作丹参地上部、根部以及根际土壤中^(13)C-光合碳比率分别为27.14%、72.80%和0.06%,连作丹参为59.38%、40.59%和0.03%。综上,连作后,丹参生长发育与次生代谢受到明显影响;光合产物向地下部的转移能力降低,导致连作丹参根部生长发育受到明显抑制;丹参光合作用的强弱是反映丹参生长状况的重要指标。The experiment was conducted by using 1-year-old potted Salvia miltiorrhiza as research object,and setting the ^(13)C pulse labeling treatment and ^(12)C normal treatment.The ^(13)C pulse labeling method was used to study the photosynthetic carbon distribution patterns of continuous and non-continuous cropping S.miltiorrhiza.The morphological and physiological differences of the plants after 30 days of labeling were recorded.The aboveground,root and rhizosphere soil of S.miltiorrhiza were collected,and their ^(13)C abundance,C isotope ratio,^(13)C atomic percentage and total ^(13)C content per unit dry weight sample were compared and analyzed to clarify the effects of continuous cropping on growth and photosynthesis of S.miltiorrhiza.The results showed that under continuous cropping conditions,the biomass and chlorophyll content of various parts of S.miltiorrhiza significantly decreased,and the activity of antioxidant enzymes increased.The content of tanshinone I,tanshinone II_A,dihydrotanshinone I and rosmarinic acid in the active ingredients decreased.The distribution ratio of photosynthetic carbon in different parts of S.miltiorrhiza was in the order of root > aboveground part > rhizosphere soil.Continuous cropping significantly affected the allocation proportion of ^(13)C photosynthetic carbon.The proportions of ^(13)C photosynthetic carbon in the aboveground part,root and rhizosphere soil of non-continuous cropping S.miltiorrhiza were 27.14%,72.80% and 0.06% respectively,while those of continuous cropping S.miltiorrhiza were 59.38%,40.59% and 0.03%,respectively.In summary,continuous cropping significantly affected the growth and secondary metabolism of S.miltiorrhiza.The photosynthetic carbon transferred into the roots decreased with the extension of time in continuous cropping S.miltiorrhiza,which limited the growth and development of the roots.The strength of photosynthesis was an important indicator reflecting the growth status of S.miltiorrhiza.

关 键 词：^(13)C脉冲标记法 光合碳 丹参 生长代谢 连作障碍 

分 类 号：S567.53[农业科学—中草药栽培]