猫血型荧光PCR检测方法的建立及应用  

Establishment and Application of Real-Time PCR for Detection of Feline Blood Type

作　　者：赵方圆 巩玉洁 赵荣茂 邓西 毕铭钰 ZHAO Fangyuan;GONG Yujie;ZHAO Rongmao;DENG Xi;BI Mingyu(Beijing Nabai Biotechnology Co.Ltd,Beijing 102600,China)

机构地区：[1]北京纳百生物科技有限公司,北京102600

出　　处：《现代畜牧科技》2024年第3期42-44,共3页Modern Animal Husbandry Science & Technology

摘　　要：猫的AB型血型系统由A型、B型和AB 3种血型组成。配型不合可能导致急性溶血性输血反应和新生儿溶血。根据TaqMan探针荧光定量分析原理,通过对猫血型决定基因胞苷-磷酸-N-乙酰神经氨酸羟基酶(CMAH)基因的4个血型相关的位点进行序列比对,设计相应引物以及探针,建立猫血型荧光PCR检测方法。该研究建立的猫血型荧光PCR检测方法与商品化的血型检测试纸条检测结果符合度达100%。此外,研究发现在北京及周边地区采集的269个猫样本中,A型血型占94.05%、B型占5.58%、AB型占0.37%。The AB blood type system in feline is composed of three blood types:A,B and AB.Mismatches may result in acute hemolytic transfusion reactions and neonatal isoerythrolysis.According to the principle of TaqMan probefluorescence quantitative analysis。four sites of the feline blood type determining gene,cytidine-phospho-N-acetylneuraminic acid hydroxylase(CMAH)gene,were aligned,and the corresponding primers and probes were designed to establish a real-time PCR detection method for feline blood type.The real-time PCR method for feline blood type detection established in this study was 100%consistent with the results of Alvedia rapid-test(LabTest A+B).In addition。in the 269 feline samples collected in Beijing and surrounding areas,the proportions of A,B and AB blood type were 94.05%,5.58%and 0.37%,respectively.

关 键 词：猫血型 CMAH基因 基因型检测 荧光PCR 

分 类 号：S829.3[农业科学—畜牧学]