铝胁迫下5种内生真菌的生长与吸收动力学特征  

Growth characteristics and absorption kinetics of five endophytic fungal strains under aluminum stress

作　　者：简灵静 张瑶锐 林晗[1,2] 谢安强 陈灿[1,2] 范海兰 JIAN Lingjing;ZHANG Yaorui;LIN Han;XIE Anqiang;CHEN Can;FAN Hailan(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Provincial Key Laboratory of Forest Ecosystem Process and Management,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]森林生态系统过程与经营福建省高校重点实验室,福建福州350002

出　　处：《森林与环境学报》2024年第2期157-163,共7页Journal of Forest and Environment

基　　金：福建省科技厅计划项目"林木-内生真菌互作对宿主生长生理的影响"(KTP21116A);福建省林业厅种业创新项目"杉木促生内生真菌资源研究与应用"(KLb21009B);福建省水保站科技支撑项目"千年桐水土保持林对植被修复与涵养功能的效应机制"(KH220045A);福州市科技计划项目"困难立地下防护林林下植被梯度恢复技术研究"(2021-P-034);福建农林大学林学高峰学科建设项目"珍贵经济树种优良种质高效培育与利用"(72202200205)。

摘　　要：为了解铝(Al)胁迫下不同内生真菌的生长情况及其对Al^(3+)的吸收动力学特征,筛选抗铝性较强的优良菌株。选取分离自南方酸性红壤区耐铝树种千年桐的5种不同内生真菌(分别隶属于拟盘多毛孢属、链格孢属、盾壳霉属、青霉属和嗜热真菌属)为供试材料,设置5个Al^(3+)浓度(0、0.555、0.746、1.111、1.852 mmol·L^(-1)),研究内生真菌的生长情况及其对Al^(3+)的吸收、吸附特征,并分析5种内生真菌的阳离子交换量和吸收动力学情况。结果表明:5种内生真菌菌株的生物量总体随着Al^(3+)浓度的升高呈现先增加后减少的趋势;当Al^(3+)浓度低于1.111 mmol·L^(-1)时,5种内生真菌均具有较强的耐铝性,当Al^(3+)浓度为1.852 mmol·L^(-1)时,5种菌株对Al^(3+)的耐受能力显著下降(P<0.05);各菌株对Al^(3+)的吸收和吸附均随着Al^(3+)浓度的升高而增加。其中,青霉属菌株的平均生长量达158 mg,其生物量显著大于其他4种菌株(P<0.05),其耐受指数、吸收动力学指标及吸附性Al^(3+)含量也相对较高,而其吸收性Al^(3+)含量均小于其他4种菌株。综合上述各项指标,相较于其他4种菌株,青霉属菌株细胞壁的吸附性Al^(3+)含量较多,能够防御Al^(3+)进入细胞内。The growth and absorption kinetics of different endophytic fungi in the presence Al^(3+)were analyzed to identify strains with strong aluminum resistance.Five strains of endophytic fungi isolated from aluminum-resistant trees in southern acidic red soils were selected for the experiment,including Pestalotiopsis,Alternaria,Coniothyrium,Penicillium,and Thermomyces spp.These strains were subjected to five different Al^(3+)concentration gradients(0,0.555,0.746,1.111,and 1.852 mmol·L^(-1)).The growth characteristics of these fungi strains and their absorption and adsorption of Al^(3+),cation exchange capacity,and absorption kinetics were studied.The results showed that the overall biomass of the five endophytic fungal strains increased,but decreased at higher Al^(3+)concentrations.All five strains had strong aluminum tolerance when the Al^(3+)concentration was below 1.111 mmol·L^(-1).However,their tolerance significantly decreased as the concentration reached 1.852 mmol·L^(-1).The absorption and adsorption of Al^(3+)by the various strains increased with increasing aluminum concentration.Compared with the other fungal species tested,Penicillium spp.had the greatest biomass(158 mg),a higher tolerance index,more favorable absorption kinetics indicators,and higher adsorptive Al^(3+)and lower absorbent Al^(3+)contents.Based on the above indicators,the cell walls of Penicillium spp.have greater adsorption of Al^(3+)compared to the cell walls of the other four strains,which may help prevent Al^(3+)entering the cell.

关 键 词：内生真菌 铝胁迫 吸收动力学 阳离子交换量 吸收和吸附 

分 类 号：S718.8[农业科学—林学]