腥掷孢酵母17wy1的分离、鉴定及对小麦白粉病防效初探  

Isolation,Identification of Golubevia albescens 17wy1 and Exploration of Its Control Effect on Wheat Powdery Mildew

作　　者：王俊美[1] 李亚红[1] 徐飞[1] 杨共强[1] 冯超红 李好海 宋玉立[1] WANG Junmei;LI Yahong;XU Fei;YANG Gongqiang;FENG Chaohong;LI Haohai;SONG Yui(Institute of Plant Protection,Henan Academy of Agricultural Sciences/Key Laboratory of Crop Integrated Pest Management of the Southern of North China,Ministry of Agriculture of the People’s Republic of China,Zhengzhou 450002,China;Henan Plant Protection and Quarantine Station,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院植物保护研究所/农业部华北南部作物有害生物综合治理重点实验室,河南郑州450002 [2]河南省植物保护检疫站,河南郑州450002

出　　处：《河南农业科学》2024年第2期101-107,共7页Journal of Henan Agricultural Sciences

基　　金：河南省小麦产业技术体系项目(HARS-22-01-G6);河南省科技攻关项目(222102110177);河南省农业科学院自主创新项目(2022ZC40);河南省重大科技专项(221100110100)。

摘　　要：采用26S rDNA序列对分离自异常生长小麦白粉菌分生孢子上的菌株17wy1进行鉴定,明确其分类地位;通过孢子萌发抑制试验、室内盆栽和田间小麦白粉病防治试验测定17wy1发酵液对小麦白粉菌分生孢子萌发的影响及对小麦白粉病的生防效果,并通过定量PCR技术分析小麦病程相关蛋白基因PR1、PR2、PR5、TaPAL的表达模式,解析17wy1的拮抗机制,为小麦白粉病的生物防治提供依据。结果表明,菌株17wy1为腥掷孢酵母(Golubevia albescens),其发酵液(5×107个/mL)对小麦白粉菌分生孢子萌发抑制率为85.1%,对室内盆栽麦苗和田间小麦白粉病的防效分别为58.5%和69.8%;喷施酵母17wy1发酵液后,室内显症麦苗体内的病程相关蛋白基因PR1、PR5被诱导显著上调表达。可见,酵母菌17wy1可抑制小麦白粉菌分生孢子萌发,对室内盆栽麦苗和田间小麦白粉病具有较好的防治作用,是小麦白粉病的潜在生防菌。An isolate 17wy1 from abnormal conidium of Blumeria graminis f.sp.tritici(Bgt)was identified by 26S rDNA sequence analysis for classification,the inhibition effect of its fermentation broth on Bgt conidium germination and the control effect of its fermentation broth on indoor pot seedling and field wheat powdery mildew were detected,and the expression pattern of wheat pathogenesis‐related genes such as PR1,PR2,PR5 and TaPAL was analyzed by RT‐qPCR in order to explain antagonistic mechanism,so as to provide a basis for 17wy1 biological control on wheat powdery mildew.The results showed that isolate 17wy1 was Golubevia albescens,its fermentation broth(5×107spores per milliliter)had 85.1%inhibition rate on Bgt conidium germination,and 58.5%control effect on wheat powdery mildew in indoor seedlings and 69.8%in field wheat,respectively.Two pathogenesis‐related protein genes PR1 and PR5 were induced and the expression was significantly up‐regulated in symptomatic indoor wheat seedlings after spraying 17wy1 fermentation broth.In conclusion,the yeast 17wy1 can inhibit the conidium germination of Bgt,has a good control effect on wheat powdery mildew both indoors and in the field,and may be a potential biocontrol agent against wheat powdery mildew.

关 键 词：小麦白粉病 腥掷孢酵母 分类鉴定 诱导抗病性 防治效果 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]