潜流式人工湿地中农村生活污水处理设施尾水的微生物特性  

Microbial characteristics of tailwater from rural domestic sewage treatment facilities in subsurface constructed wetlands

作　　者：陈曙平 卢原 张文艺 李乔 吴科 吴金海 金林飞 CHEN Shu-ping;LU Yuan;ZHANG Wen-yi;LI Qiao;WU Ke;WU Jin-hai;JIN Lin-fei(Changzhou Yurun Water Co.,Ltd.,Changzhou 213164,Jiangsu,China;School of Environmental and Safety Engineering,Changzhou University,Changzhou 213164,Jiangsu,China;Jiangsu KTE Group Co.,Ltd.,Changzhou 213102,Jiangsu,China)

机构地区：[1]常州禹润水务有限公司,江苏常州213164 [2]常州大学环境与安全工程学院,江苏常州213164 [3]江苏今创环境集团有限公司,江苏常州213102

出　　处：《湖北农业科学》2024年第2期219-223,共5页Hubei Agricultural Sciences

基　　金：江苏省科技支撑计划项目(BE20207611)。

摘　　要：农村生活污水经A1/O1/A2/O2设施处理后流入潜流式人工湿地(简称人工湿地),为揭示其生物降解特性,通过脲酶活性和微生物扩增子测序对人工湿地微生物特性进行分析。结果表明,人工湿地进水处的脲酶活性高于出水处的脲酶活性,出水处的脲酶活性与总氮去除率呈显著正相关,出水处脲酶活性越高,总氮的去除效果越好;微生物扩增子测序表明,人工湿地的优势菌门有变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes),由进水处到出水处变形菌门和拟杆菌门相对丰度分别下降8.41个百分点和12.14个百分点,厚壁菌门上升12.91个百分点;人工湿地进水处、出水处脱氮菌属含量较大,相对丰度分别为20.51%、14.18%,其中,具有异养硝化反硝化功能的菌属也相对丰富,分别为8.54%、7.44%。Rural domestic sewage was treated by A1/O1/A2/O2 facilities and flowed into the subsurface constructed wetland(abbreviated as constructed wetland).To reveal its biodegradation characteristics,the microbial characteristics of constructed wetland were analyzed through urease activity and microbial amplicon sequencing.The results showed that the urease activity at the inlet of the constructed wetland was higher than that at the outlet.The urease activity at the outlet was significantly positively correlated with the total nitrogen removal rate.The higher the urease activity at the outlet,the better the total nitrogen removal effect;microbial amplicon sequencing showed that the dominant bacterial phyla in constructed wetland were Proteobacteria,Bacteroidetes,and Firmicutes.The relative abundance of Proteobacteria and Bacteroidetes decreased by 8.41 percentage points and 12.14 percentage points respectively from the inlet to the outlet,while Firmicutes increased by 12.91 percentage points;the content of denitrifying bacteria in the inlet and outlet of the constructed wetland was relatively high,with relative abundances of 20.51%and 14.18%,respectively.Among them,the bacteria with heterotrophic nitrification and denitrification functions were also relatively abundant,with 8.54%and 7.44%,respectively.

关 键 词：A1/O1/A2/O2 潜流式人工湿地 农村生活污水处理设施 尾水 微生物 脲酶活性 微生物扩增子测序 

分 类 号：X703[环境科学与工程—环境工程]