荔枝GRF基因家族的全基因组鉴定及表达分析  

Genome-wide identification and expression analysis of GRF gene family in litchi

作　　者：董晨[1] 郑雪文 王弋[1] 全振炫 李伟才[1] DONG Chen;ZHENG Xuewen;WANG Yi;QUAN Zhenxuan;LI Weicai(Institute of South Subtropical Crops,Chinese Academy of Tropical Agricultural Science/Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture and Rural Affairs,Zhanjiang,Guangdong 524091,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室,广东湛江524091

出　　处：《热带生物学报》2024年第2期190-197,共8页Journal of Tropical Biology

基　　金：海南省自然科学基金项目(321MS077);国家重点研发计划项目(2019YFD1000900);中央级公益性科研院所基本科研业务专项(1630042022004,1630062023017);国家荔枝龙眼产业技术体系项目(CARS-32-20)。

摘　　要：为揭示荔枝(Litchi chinensis)GFR基因的功能,对荔枝生长调控因子(LcGRF)基因家族进行了全基因组鉴定和分析,并研究其在荔枝中的表达模式。基于荔枝基因组数据库,使用生物信息学软件对LcGRFs家族成员进行全基因组鉴定,分析基本理化性质、染色体定位、基因结构、进化关系、蛋白保守基序、顺式作用元件和时空表达情况进行系统分析。共获得12个GRF基因,不均匀地分布在10条染色体上,内含子2~5个。蛋白保守基序分析发现,荔枝GRF蛋白均含有保守的motif1(WRC)和motif2(QLQ),进化分析LcGRF划分为5个亚家族,LcGRFs启动子上存在大量的光、植物激素、非生物胁迫响应以及生长发育相关的顺式作用元件。不同转录组表达模式结果显示,各个组织中呈现出多样化的表达特征,表明不同成员可能在荔枝不同生长发育过程中发挥调控作用,参与调节荔枝的生长发育。研究显示,荔枝GRF家族成员有12个,划分为5个亚家族。In order to reveal the functions of litchi GFR gene,the whole genome of litchi growth-regulating factor(LcGRF)gene family was identified and analyzed,and its expression patterns in different tissues of litchi were studied.Based on the litchi genome database,bioinformatics software was used to conduct genome-wide identification of members of the LcGRFs family,an analysis was made of basic physical and chemical properties,chromosome localization,gene structure,evolutionary relationships,protein conserved motifs,cis-acting elements,and spatiotemporal expression for systematic analysis.A total of 12 GRF genes were obtained from litchi,which were unevenly distributed on 10 chromosomes,with 2-5 introns.The analysis of the conserved motifs of the GRF protein found that all the GRF proteins in litchi contained conservative motifs of motif 1(WRC)and motif 2(QLQ).According to evolutionary analysis,LcGRF was divided into five subfamilies.There were a large number of cis-acting elements related to light,plant hormones,abiotic stress responses and growth and development on the promoter of LcGRFs.The results of different transcriptome expression patterns show that LcGRFs exhibits diverse expression characteristics in various tissues,indicating that different members may play regulatory roles in different growth and development processes of litchi,participating in regulating the growth and development of litchi.The analysis shows that there are 12 members of the litchi GRF family,which are divided into 5 subfamilies.

关 键 词：荔枝 生长调控因子 生物信息 表达模式 

分 类 号：S667.1[农业科学—果树学] Q943.2[农业科学—园艺学]