冠层活性相关性状全基因组关联分析及其对产量性状遗传效应的解析  

Genome-Wide Association Studies for Canopy Activity Related Traits and Its Genetic Effects on Yield-Related Traits

作　　者：李法计 程敦公[1] 余晓丛 闻伟锷 刘金栋[4] 翟胜男 刘爱峰[1] 郭军[1] 曹新有[1] 刘成[1] 宋健民[1] 刘建军[1] 李豪圣[1] LI FaJi;CHENG DunGong;YU XiaoCong;WEN WeiE;LIU JinDong;ZHAI ShengNan;LIU AiFeng;GUO Jun;CAO XinYou;LIU Cheng;SONG JianMin;LIU JianJun;LI HaoSheng(Crop Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Research Center of Wheat and Maize/Key Laboratory of Wheat Biology and Genetics and Breeding in Northern Huang-Huai River Plain,Ministry of Agriculture and Rural Affairs/Shandong Technology Innovation Center of Wheat/Jinan Key Laboratory of Wheat Genetic Improvement,Jinan 250100;College of Agricultral and Biological Engineering(College of Tree Peony),Heze University,Heze 274015,Shandong;Department of Cell Biology,Zunyi Medical University,Zunyi 563099,Guizhou;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]山东省农业科学院作物研究所/小麦玉米国家工程研究中心/农业农村部黄淮北部小麦生物学与遗传育种重点实验室/山东省小麦技术创新中心/济南市小麦遗传改良重点实验室,济南250100 [2]菏泽学院农业与生物工程学院(牡丹学院),山东菏泽274015 [3]遵义医科大学细胞生物学教研室,贵州遵义563099 [4]中国农业科学院作物科学研究所,北京100081

出　　处：《中国农业科学》2024年第4期627-637,共11页Scientia Agricultura Sinica

基　　金：山东省重点研发计划(重大科技创新工程)(2021LZGC009);泰山产业领军人才工程(LJNY202006);国家现代农业产业技术体系(CARS-03-6);2023年山东省农业科学院科技创新工程(CXGC2023A01);济南市“新高校20条”资助项目(202228067)。

摘　　要：【目的】冠层活性是反映作物生长发育的重要指标,发掘小麦冠层活性相关基因并分析其与产量性状的关系,为解析产量遗传结构和辅助育种提供理论支撑。【方法】以166份国内外小麦品种为材料,将其种植于河南安阳和安徽濉溪,结合已构建的包含326570个来源于小麦90K和660K SNP芯片标记的高密度整合物理图谱,对苗期和花后10 d归一化植被指数(NDVI-S和NDVI-10)及花后10 d旗叶叶绿素含量(Chl-10)进行关联分析,并与前期利用相同材料得到的产量相关性状结果进行比较。【结果】NDVI-S、NDVI-10和Chl-10在基因型、环境及基因型×环境互作间变异方差均达极显著(P<0.01)差异,广义遗传力(hb2)分别为0.81、0.81和0.91。分别检测到13、12和15个与NDVI-S、NDVI-10和Chl-10显著相关的位点,其中,有12、11和12个为新位点,5个位点与2个以上性状有关。166份小麦品种含有NDVI-S、NDVI-10和Chl-10优异等位基因数变幅分别为4-11、3-11和4-12,且随着优异等位基因数目增加其对应表型值呈递增趋势。NDVI-S与千粒重、粒长和粒宽显著正相关(P<0.01);Chl-10与产量和旗叶宽显著正相关(P<0.01),与单位面积穗数、株高和穗下节长显著负相关(P<0.01)。检测到7个同时与产量和冠层活性相关的多效性位点。【结论】NDVI-S可用于品种产量性状的选择,检测到的稳定位点和多效性位点可在开发标记后用于育种材料的检测。【Objective】Canopy activity is an important indicator of wheat growth and development.Identification the loci for canopy activity related traits and their relationships with grain yield(GY)related traits can provide theoretical support for the dissection of genetic structure of yield trait and assisted wheat breeding.【Method】A total of 166 wheat varieties originating from both domestic and international sources were planted in Anyang of Henan province and Suixi of Anhui province in cropping seasons.With the integrated physical map containing 326570 SNP markers from the wheat 90K and 660K chips,genome-wide association studies for normalized difference vegetation index at seedling stage(NDVI-S)and 10 days after flowering(NDVI-10),and chlorophyll content in flag leaf at 10 days after flowering(Chl-10)were carried out.The results were compared with the previous study for GY related traits using the same material.【Result】Analysis of variance(ANOVA)showed highly significant effects(P<0.01)of genotypes,environments and genotype×environment interactions on NDVI-S,NDVI-10 and Chl-10,with broad-sense heritabilities(h2 b)of 0.81,0.81 and 0.91,respectively.Thirteen,12 and 15 loci were detected to be significantly correlated with NDVI-S,NDVI-10 and Chl-10,respectively,among which 12,11 and 12 were new,and five loci were associated with two or more traits.The number of favorable alleles for NDVI-S,NDVI-10 and Chl-10 ranged from 4 to 11,3 to 11 and 4 to 12,respectively,in the 166 wheat varieties,and the phenotypic values increased with the accumulation of favorable alleles.NDVI-S showed significant(P<0.01)and positive correlations with thousand-kernel weight,kernel length and kernel width.Chl-10 was significant positively correlated with GY and flag leaf width(P<0.01),whereas significant negatively correlated with spike number per unit area,plant height and uppermost internode length(P<0.01).Seven pleiotropic loci were detected co-related with both GY and canopy activity related traits.【Conclusion】NDVI-S can be

关 键 词：小麦 冠层活性 产量 归一化植被指数 叶绿素含量 关联分析 

分 类 号：S512.1[农业科学—作物学]