皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响  

Effects of the proliferation,migration and apoptosis of AHVAC-Ⅰon gastric cancer MKN-28 cells

作　　者：黄小梅[1] 支慧 陈浩[1] 卢林明[1] 朱晓群[1] 王丽珍[1] 周珏[1] 庞金金 许金亮 HUANG Xiaomei;ZHI Hui;CHEN Hao;LU Linming;ZHU Xiaoqun;WANG Lizhen;ZHOU Jue;PANG Jinjin;XU Jinliang(Department of Pathology,Wannan Medical College,Wuhu 241002,Anhui,China;Clinical Medicine of Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院病理解剖学教研室,安徽芜湖241002 [2]皖南医学院临床医学院,安徽芜湖241002

出　　处：《中国临床药理学与治疗学》2024年第3期270-276,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省教育厅自然科学研究重点项目(KJ2020A0611);皖南医学院大学生科研资助金项目(WK2020S01);活性生物大分子研究安徽省重点实验室自主研究课题(LAB202004);安徽省高等学校质量工程项目(S202010368107)。

摘　　要：目的:探讨皖南蝮蛇毒抑瘤组分-Ⅰ(agkistrodon halys venom antitumor componentⅠ,AHVAC-Ⅰ)对胃癌MKN-28细胞生物学行为的影响作用。方法:不同实验浓度(5、10和15μg/mL)AHAVC-Ⅰ处理胃癌细胞MKN-2824 h后,采用细胞增殖和毒性检测(cell counting kit-8,CCK-8)法检测细胞增殖活力;划痕实验和Tanswell小室检测细胞迁移能力;激光共聚焦显微镜(annexin VmCherry/DAPI双染)和流式细胞术(annexin VFITC/PI双染)检测细胞凋亡;Western blot检测细胞凋亡相关蛋白Caspease-3的表达水平。结果:AHVAC-Ⅰ各浓度组分别与正常对照组相比结果显示,随着AHVAC-Ⅰ浓度的增加,MKN-28细胞增殖活力逐渐下降(P<0.01),细胞迁移能力逐渐被抑制(P<0.01),细胞凋亡率增高(P<0.05),凋亡相关蛋白Caspease-3表达上调(P<0.01)。结论:AHVAC-Ⅰ抑制胃癌细胞MKN-28增殖和迁移,诱导其凋亡。AIM:To investigate the effects of agkis-trodon halys venom anti-tumor componentⅠ(AHVAC-Ⅰ)on the biological behavior of gastric cancer MKN-28 cells.METHODS:Gastric cancer MKN-28 cells were treated with the experimental concentrations(5,10,15μg/mL)of AHAVC-Ⅰfor 24 h.Cell proliferation and toxicity assay(cell counting kit-8,CCK-8)was used to detect the inhibition rates of the cells in different concentrations of AHVAC-Ⅰ.The migration ability of the cells was evaluated by wound-healing and Transwell assay.The apoptosis were observed by laser confocal microscopy with annexin V-mCherry/DAPI double staining,and the apoptosis rates were analyzed by flow cytometry with annexin V-FITC/PI double fluorescence staining.The protein level of Caspease-3 was determined by Western blot.RESULTS:Compared with normal control group,the results of AHVAC-Ⅰconcentration groups showed that with the increase of AHVAC-Ⅰconcentration,the proliferative activity of MN-28 cells decreased gradually(P<0.01),the cell migration ability decreased gradually(P<0.01),and the cell apoptosis rate increased(P<0.05).The expression of apoptosis-related protein Caspease-3 was up-regulated(P<0.01).CONCLUSION:AHVAC-Ⅰinhibits proliferation and migration of gastric cancer MSN-28 cells and induces apoptosis.

关 键 词：AHVAC-Ⅰ 胃癌 细胞增殖 细胞迁移 细胞凋亡 

分 类 号：R965.2[医药卫生—药理学]