异菌脲降解菌YJN1-1的分离鉴定及生长特性研究  

Isolation,Identification and Growth Characteristics of Iprodione Degradation Bacterium YJN 1-1

作　　者：周海龙 卜文斌 郭玉珩 李玙卓 江烨 刘虎虎[1] 潘虎 ZHOU Hailong;BU Wenbin;GUO Yuheng;LI Yuzhuo;JIANG Ye;LIU Huhu;PAN Hu(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha Hunan 410128;Institute of Agricultural Quality Standards and Testing,Tibet Academy of Agriculture and Animal Husbandry Sciences,Lhasa Tibet 850032)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]西藏自治区农牧科学院农业质量标准与检测研究所,西藏拉萨850032

出　　处：《现代农业科技》2024年第6期152-156,共5页Modern Agricultural Science and Technology

基　　金：国家自然科学基金地区合作基金(32060025);湖南农业大学大学生创新训练项目(s202010537078);湖南省大学生创业训练计划项目(S202110537006X)。

摘　　要：为了解决西藏自治区土壤异菌脲残留问题,本文从西藏自治区长期施用异菌脲的土壤中分离得到一株异菌脲降解菌YJN1-1,对其进行了形态特征、生理生化特性和16S rRNA基因序列同源性分析,同时通过单因素试验研究了其培养条件。结果表明,菌株YJN1-1鉴定为苍白杆菌属(Ochrobactrum sp.);菌株YJN1-1的最适生长pH值、最适培养温度和最适摇床转速分别为7.0、37℃和210 r/min。In order to solve the problem of iprodione residue in the soil of Tibet Autonomous Region,an iprodione degrading bacterium YJN1-1 was isolated from the soil of Tibet Autonomous Region where iprodione was applied for a long time,and its morphological characteristics,physiological and biochemical characteristics and 16S rRNA gene sequence homology were analyzed.At the same time,its culture conditions were studied through single factor experiments.The results showed that strain YJN1-1 was identified as Ochrobactrum sp.;the optimal growth pH,culture temperature,and shaker rotation speed for strain YJN1-1 were 7.0,37℃and 210 r/min,respectively.

关 键 词：土壤 异菌脲残留 生物降解 异菌脲降解菌 培养条件 

分 类 号：S172[农业科学—农业基础科学]