厚朴挥发油纳米乳凝胶的制备及其对溃疡性结肠炎小鼠的药效评价  

Preparation of Magnoliae Officinalis Cortex volatile oil nanogel and its efficacy evaluation on ulcerative colitis mice

作　　者：张倩[1] 刘芳[1] 张芮苑 邓鸿丹 杨璐萍 任虹[1] ZHANG Qian;LIU Fang;ZHANG Ruiyuan;DENG Hongdan;YANG Luping;REN Hong(State Key Laboratory of Traditional Chinese Medicine Resources with Southwest Characteristics,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学西南特色中药资源国家重点实验室,四川成都611137

出　　处：《中草药》2024年第3期746-756,共11页Chinese Traditional and Herbal Drugs

基　　金：四川省自然科学基金项目(2023NSFSC0661);成都市科技局项目(2022-YF05-01112-SN)。

摘　　要：目的 制备厚朴Magnoliae Officinalis Cortex(MOC)挥发油纳米乳凝胶(MOC volatile oil nanogel,MNG),评价其结肠靶向释放性能,并考察其对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导溃疡性结肠炎(ulcerative colitis,UC)小鼠的治疗作用。方法 采用自乳化法制备厚朴挥发油纳米乳(MOC volatile oil nanoemulsion,MN),并对其形貌、粒径、电位等理化性质进行表征;以成胶时间为指标优选透明质酸和海藻酸钠的质量浓度,将最优比的透明质酸和海藻酸钠溶于MN中形成MNG,对得到的MNG进行理化性质表征,并评价其结肠靶向和对DSS诱导的UC小鼠的治疗作用。结果 所制得的MN外观澄清、透明、微泛蓝光,透射电镜观察纳米乳呈球形,平均粒径为(61.56±1.67)nm,ζ电位为(-21.37±0.45)m V,包封率为(85.25±0.50)%;在最佳MN配方的基础上,以海藻酸钠和透明质酸质量浓度分别为30 mg/mL和40mg/mL作为凝胶基质,制得最佳MNG;药效学实验证明MNG对UC小鼠具有较好的治疗作用。结论 成功制备MNG,提高厚朴挥发油稳定性的同时,使其具有结肠靶向释放能力。Objective To prepare Houpo(Magnoliae Officinalis Cortex,MOC)volatile oil nanogel(MNG),evaluate its colon targeted release performance,and investigate its therapeutic effect on dextran sodium sulfate(DSS)induced UC mice.Methods MOC volatile oil nanoemulsion(MN)was prepared by self emulsification method,and its morphology,particle size,potential and other physicochemical properties were characterized;The concentration of hyaluronic acid and sodium alginate was optimized with the gelation time as the index.The optimal ratio of hyaluronic acid and sodium alginate powder was dissolved in the MN to form MNG.The physical and chemical properties of the obtained MNG were characterized,and its colon targeting and therapeutic effects on DSS induced UC mice were evaluated.Results The prepared MN has a clear,transparent,and slightly bluish appearance.According to transmission electron microscopy,the nanoemulsion is spherical with an average particle size of(61.56±1.67)nm,a potential of(-21.37±0.45)mV,and an encapsulation efficiency of(85.25±0.50)%;On the basis of the best formula of MN,the optimal MNG was prepared with the mass concentration of sodium alginate and hyaluronic acid of 30 mg/mL and 40 mg/mL respectively as the gel matrix;The pharmacodynamic experiments had shown that MNG has a good therapeutic effect on UC mice.Conclusion The MNG was successfully prepared,which improved the stability of MOC volatile oil and made it have colon targeted release capacity.

关 键 词：厚朴挥发油 溃疡性结肠炎 结肠靶向 纳米乳 纳米乳凝胶 稳定性 

分 类 号：R283.6[医药卫生—中药学]