γ-氨基丁酸对怀玉山三叶青低温胁迫下试管苗生长及相关生理代谢的影响  

Effects ofγ-Aminobutyric Acid on the Growth and Related Physiological Metabolism of Plantlets of Tetrastigma hemsleyanum Diels et Gilg From Huaiyu Mountain Under Low Temperature Stress

作　　者：洪森荣[1,2,3,4] 余锾媛 彭睿羚 刘雯莉 胡藤鑫 王玉婷 HONG Senrong;YU Huanyuan;PENG Ruiling;LIU Wenli;HU Tengxin;WANG Yuting(College of Life Sciences,Shangrao Normal University,Shangrao 334001 Jiangxi,China;Shangrao Agricultural Technology Innovation Research Institute,Shangrao 334001 Jiangxi,China;Key Laboratory of Protection and Utilization of Medicinal and Edible Plant Resources in Shangrao City,Shangrao 334001 Jiangxi,China;Shangrao Tetrastigma hemsleyanum Diels et Gilg Conservation and Utilization Technology Innovation Center,Shangrao 334001 Jiangxi,China)

机构地区：[1]上饶师范学院生命科学学院,江西上饶334001 [2]上饶农业技术创新研究院,江西上饶334001 [3]上饶市药食同源植物资源保护与利用重点实验室,江西上饶334001 [4]上饶市三叶青保育与利用技术创新中心,江西上饶334001

出　　处：《中药新药与临床药理》2024年第1期132-140,共9页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金资助项目(31960079);2022年上饶市科技专项项目(饶科发[2023]5号社发类,2022A008);江西省科技厅重点研发计划一般项目(20202BBG73010);江西省教育厅科学技术研究项目(GJJ201704);上饶市科技局平台载体建设项目(2020J001);上饶师范学院2023年大学生创新创业训练计划项目(2023-PX-17)。

摘　　要：目的探讨γ-氨基丁酸(GABA)对怀玉山三叶青低温胁迫下试管苗生长及相关生理代谢的影响,为其在怀玉山三叶青仿野生栽培中的防冻抗寒应用提供依据。方法以低温胁迫下GABA处理的怀玉山三叶青试管苗为研究对象,采用植物组织培养法测定其生长指标,植物生理生化法测定其生理指标,实时荧光定量聚合酶链反应(qRT-PCR)法检测其查尔酮合酶和黄酮醇合酶基因的相对表达量。结果与未添加GABA的对照组相比,培养基中添加0.5 g·L^(-1)GABA,怀玉山三叶青2个栽培种“怀玉1号”和“怀玉2号”试管苗的增殖系数、株高、鲜质量、干质量及组织含水量均达到最高值;可以显著提高其可溶性蛋白(SP)、可溶性糖(SS)、脯氨酸(Pro)、还原型谷胱甘肽(GSH)、抗坏血酸(AsA)、吲哚乙酸(IAA)、玉米素核苷(ZR)、赤霉素(GA3)、茉莉酸(JA)的含量,以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)的活性和查尔酮合酶(CHS)基因的相对表达量(RQ值);显著降低其相对电导率、脱落酸(ABA)、过氧化氢(H_(2)O_(2))、丙二醛(MDA)的含量,O_(2)·^(-)产生速率,以及黄酮醇合酶(FLS)基因的相对表达量。低温胁迫下GABA处理的怀玉山三叶青试管苗的生长与IAA、ZR、GA3、JA、SS、AsA、GSH的含量和CHS基因表达呈正相关。结论适当浓度的GABA可以调控内源植物激素和渗透调节物质含量、抗氧化物质含量以及次生代谢物关键基因表达,以促进低温胁迫下怀玉山三叶青试管苗的生长。Objective To explore the effect ofγ-aminobutyric acid(GABA)on the growth and related physiological metabolism of plantlets of Tetrastigma hemsleyanum Diels et Gilg from Huaiyu Mountain under low temperature stress,which provides a basis for its application of anti-freezing and cold resistance in the wild-imitation cultivation of T.hemsleyanum Diels et Gilg from Huaiyu mountain.Methods Plantlets of T.hemsleyanum Diels et Gilg from Huaiyu Mountain,which were treated with GABA under low temperature stress,were selected as research objects.The growth indicators,physiological indexes,the relative expression levels of chalcone synthase and flavonol synthase genes were measured using plant tissue culture,physiological and biochemical methods and qRT-PCR,respectively.Results Compared with the control group without GABA,the proliferation coefficient,plant height,fresh weight,dry weight,and tissue water content of the two cultivated plantlets of T.hemsleyanum Diels et Gilg from Huaiyu Mountain(“Huaiyu 1”and“Huaiyu 2”)reach their maximum value when 0.5 g·L^(-1)GABA was added to the culture medium.Moreover,content of soluble protein(SP),soluble sugar(SS),proline(Pro),reduced glutathione(GSH),ascorbic acid(AsA),indole acetic acid(IAA),zeatin nucleoside(ZR),gibberellin(GA3),jasmonic acid(JA),the activity of superoxide dismutase(SOD),peroxidase(POD),ascorbic acid peroxidase(APX)and glutathione reductase(GR),and the relative expression level(RQ value)of chalcone synthase(CHS)gene were significantly increased.Moreover,relative conductivity,content of abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA),rate of O_(2)·^(-)production,and relative expression levels(RQ value)of flavonol synthase(FLS)gene were obviously decreased.The growth of plantlets of T.hemsleyanum Diels et Gilg from Huaiyu Mountain,which were treated with GABA under low temperature stress,was positively correlated with the contents of IAA,ZR,GA3,JA,SS,AsA,GSH,and the expression of CHS genes.Conclusion Appropriate concentration of GABA

关 键 词：氨基丁酸 怀玉山三叶青 低温胁迫 试管苗 生长 生理代谢 

分 类 号：R282.2[医药卫生—中药学]