酶法制备野黄芩素的工艺研究  

Research on the Technology for Enzymatic Preparation of Scutellarein

作　　者：程雨婕 刘云华[2] 黄志芳[2] 陈燕[2] 刘玉红[2] 易进海 CHENG Yujie;LIU Yunhua;HUANG Zhifang;CHEN Yan;LIU Yuhong;YI Jinhai(School of Pharmacy of Chengdu University of Traditional Chinese Medicine,Chengdu 611130 Sichuan,China;Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica,Sichuan Academy of Chinese Medicine sciences,Chengdu 610041 Sichuan,China)

机构地区：[1]成都中医药大学药学院,四川成都611130 [2]四川省中医药科学院中药材品质及创新中药研究四川省重点实验室,四川成都610041

出　　处：《中药新药与临床药理》2024年第2期274-279,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：四川省科技计划(重点研发)项目(2022YFS0640);四川省科研院所科技成果转化项目(2021JDZH0018)。

摘　　要：目的应用黄芩茎叶葡萄糖醛酸水解酶(Scutellaria baicalensis stems and leaves glucuronic hydrolase,sbsl GUS)酶解灯盏花中野黄芩苷制备野黄芩素,经分离纯化获得高纯度的野黄芩素提取物。方法采用正交试验优选灯盏花提取工艺参数;以野黄芩苷酶解转化率为指标,对酶用量、酶解pH值、温度、时间、抗氧化剂等进行考察,优选野黄芩素制备工艺参数;采用乙醇提取、活性炭脱色和分步结晶精制纯化粗提物。结果灯盏花提取工艺为灯盏花切段,加10倍水煎煮提取2次,每次1 h。野黄芩素制备工艺为sbsl GUS提取液和灯盏花水煎液加入量(以生药计)比为1∶10,加0.5%偏重亚硫酸钠,酶解pH值约6.0,温度约45℃,时间20 h,得到含量大于60%的野黄芩素粗提物。经分步结晶对粗提物进行纯化,用80%乙醇回流提取,活性炭脱色,浓缩、析晶,得到含量大于85%的野黄芩素提取物。结论该工艺应用sbsl GUS酶解技术制备野黄芩素,生产简便可行,为野黄芩素的生产制备提供了新的途径。Objective Scutellaria baicalensis stems and leaves glucuronic hydrolase(sbslGUS)was used to enzymatically hydrolyze scutellarin in Erigeron breviscapus(Vant.)Hand.Mazz.to prepare scutellarein,and the high-purity scutellarein was obtained through separation and purification.Methods Orthogonal experiments were used to optimize the process parameters for the extraction of Erigeron breviscapus(Vant.)Hand.Mazz..Using the rate of enzymatic hydrolysis conversion of scutellarin as the index,the amount of enzyme,pH,temperature,time and antioxidant were investigated,and the preparation process parameters of scutellarein were optimized.Ethanol extraction,activated carbon decolorization,and fractional crystallization were used to purify the crude extract.Results The extraction process was determined to be:segments of Erigeron breviscapus were decocted twice with 10 times water for 1 hour each time.The preparation process of scutellarein was as follows:the amount of sbslGUS extract and Erigeron breviscapus decoction was 1∶10 based on crude drugs,0.5%sodium metabisulfite was added,pH value was about 6.0,the temperature was about 45℃,and the time was 20 hours.The crude extract of scutellarein with the content more than 60%was obtained.The crude extract was purified by fractional crystallization,refluxed with 80%ethanol,decolorized with activated carbon,concentrated and crystallized,and the scutellarein extract with content more than 85%was obtained.Conclusion sbslGUS enzymatic hydrolysis technology,which was used to prepare scutellarein,is simple and feasible.This study provides a new way for the manufacture of scutellarein.

关 键 词：灯盏花 野黄芩苷 野黄芩素 黄芩茎叶葡萄糖醛酸水解酶 酶解转化 工艺研究 

分 类 号：R284.2[医药卫生—中药学]