不同病原学方法检测野鼠日本血吸虫感染效果比较  

Comparison of the efficiency of different etiological assays for detection of Schistosoma japonicum infections in wild mice

作　　者：许晓娟[1] 陈雪峰 吴凡 吴晨阳 刘婷 代波[1] 汪天平[1] 张世清[1] XU Xiaojuan;CHEN Xuefeng;WU Fan;WU Chenyang;LIU Ting;DAI Bo;WANG Tianping;ZHANG Shiqing(Anhui Provincial Institute of Schistosomiasis Control,Hefei,Anhui 230601,China;Shitai County Center for Disease Control and Prevention,Anhui Province,China;Wuhu Municipal Station for Endemic Disease Control,Anhui Province,China;Guichi District Station of Schistosomiasis Control,Chizhou City,Anhui Province,China)

机构地区：[1]安徽省血吸虫病防治研究所,安徽合肥230601 [2]安徽省池州市石台县疾病预防控制中心 [3]安徽省芜湖市地方病防治站 [4]安徽省池州市贵池区血吸虫病防治站

出　　处：《中国血吸虫病防治杂志》2023年第6期573-582,589,共11页Chinese Journal of Schistosomiasis Control

基　　金：安徽省第十四批“115”产业创新团队资助项目。

摘　　要：目的比较不同病原学方法在野鼠日本血吸虫感染检测中的应用价值,为血吸虫病传播风险评估提供技术支持。方法2022年10—11月,于安徽省血吸虫病流行区选取有钉螺分布的环境采用夹夜法捕捉野鼠。采用肝脏组织压片镜检法、肠系膜组织镜检法、肝脏组织匀浆镜检法、肝脏组织匀浆孵化法、改良加藤厚涂片法(Kato⁃Katz法)和粪便毛蚴孵化法等6种方法单独或联合应用检测野鼠日本血吸虫感染,任一种方法检出日本血吸虫虫卵或毛蚴即判为血吸虫感染阳性,比较不同方法检测野鼠日本血吸虫感染敏感度。结果共捕获野鼠1703只,其中366只(21.49%)野鼠日本血吸虫感染阳性。6种方法检测野鼠日本血吸虫感染率(Q=529.33,P<0.001)及敏感度(χ^(2)=527.78,P<0.001)差异均具有统计学意义。肝脏组织压片镜检法联合肠系膜组织镜检法、肝脏组织压片镜检法联合肝脏组织匀浆镜检法及联合应用肝脏组织压片镜检法、肠系膜组织镜检法、肝脏组织匀浆镜检法及Kato⁃Katz法等4种方法检测野鼠日本血吸虫感染敏感度较高,分别为86.61%、87.16%和97.27%。结论不同病原学方法检测野鼠日本血吸虫感染敏感度存在差异,肝脏组织压片镜检法联合肠系膜组织镜检法、肝脏组织压片镜检法联合肝脏组织匀浆镜检法具有潜在现场应用价值。Objective To compare the efficiency of multiple etiological techniques for detection of Schistosoma japonicum in⁃fections in wild mice,so as to provide technical supports to assessment of schistosomiasis transmission risk.Methods Wild mice were captured with baited traps at night in Oncomelania hupensis snail⁃infested settings in schistosomiasis⁃endemic foci of Anhui Province from October to November,2022.S.japonicum infections were detected in wild mice using microscopy of mouse liver tissues,microscopy of mouse mesenteric tissues,microscopy of mouse liver tissue homogenates,miracidial hatching test of mouse liver tissue homogenates,Kato⁃Katz technique and miracidial hatching test of mouse stool samples alone and in combina⁃tions.Identification of S.japonicum eggs or miracidia by any of these six assays was defined as an infection.The sensitivity of six assays alone or in combinations was compared for detection of S.japonicum infections in wild mice.Results A total of 1703 wild mice were captured,with 366 wild mice detected positive for S.japonicum(21.49%).There were significant differences in the prevalence of S.japonicum infections in wild mice by six assays(Q=529.33,P<0.001)and in the sensitivity of six assays for detection of S.japonicum infections in wild mice(χ^(2)=527.78,P<0.001).In addition,the combination of microscopy of mouse liver tissues and mesenteric tissues,combination of microscopy of mouse liver tissues and liver tissue homogenates and combination of microscopy of mouse liver tissues,microscopy of mesenteric tissues,microscopy of liver tissue homogenates and Kato⁃Katz technique showed 86.61%,87.16%and 97.27%sensitivities for detection of S.japonicum infections in wild mice,re⁃spectively.Conclusions Diverse etiological assays show various efficiencies for detection of S.japonicum infections in wild mice.Combination of microscopy of mouse liver tissues and microscopy of mesenteric tissues,and combination of microscopy of mouse liver tissues and microscopy of liver tissue homogenate

关 键 词：日本血吸虫 野鼠 病原学方法 检测效能 

分 类 号：R383.24[医药卫生—医学寄生虫学]