灵芝孢子油及添加维生素E灵芝孢子油对NRK-52E细胞氧化损伤的作用比较  

Comparison of the Effects of Ganoderma lucidum Spore Oil and Vitamin E Adding Ganoderma lucidum Spore Oil on Oxidative Damage of NRK-52E Cells

作　　者：张玉坤 周俊甫 潘新华 杨宝学[1] ZHANG Yukun;ZHOU Junfu;PAN Xinhua;YANG Baoxue(Department of Pharmacology,School of Basic Medicine,Peking University,Beijing 100191,China;Jiangxi Xiankelai Biotechnology Co.,Ltd.,Jiujiang 332000,China;Chongqing Key Laboratory for the Development and Utilization of Genuine Medicinal Materials in the Three Gorges Reservoir Area,Chongqing Three Gorges Medical College,Wanzhou 404120,China)

机构地区：[1]北京大学基础医学院药理系,北京100191 [2]江西仙客来生物科技有限公司,江西九江332000 [3]重庆三峡医药高等专科学校三峡库区道地药材开发与利用重庆市重点实验室,重庆万州404120

出　　处：《中国食用菌》2024年第1期48-54,共7页Edible Fungi of China

基　　金：国家自然科学基金青年项目(82304493);重庆三峡医药高等专科学校重大项目(XJ2022002303)。

摘　　要：灵芝孢子油是从灵芝孢子粉中提取的油状脂质物,其中的不饱和脂肪酸和三萜类灵芝酸是其主要活性成分。是由于其中的不饱和脂肪酸易氧化挥发,研究人员通过添加维生素E以求解决难题,但目前对添加维生素E的灵芝孢子油抗氧化应激活性的系统研究未见报道。因此选用灵芝孢子油(GLSO)及添加5%维生素E的灵芝孢子油(GLSO+VE)2种样品,作用于H2O2处理的大鼠肾小管上皮NRK-52E细胞,以评价、比较对氧化应激损伤的保护作用。结果表明,GLSO和GLSO+VE均能显著性增加NRK-52E细胞存活率,提高抗氧化酶的活性,降低氧化产物水平。比较可知添加维生素E可显著降低灵芝孢子油的细胞毒作用,并在一定程度上增加其抗氧化活性。此次试验初步证实了灵芝孢子油中添加维生素E对抗细胞氧化损伤的增强作用,为添加维生素E以增加灵芝孢子油稳定性的研究提供更多理论依据和支撑数据。Ganoderma lucidum spore oil is an oily lipid substance extracted from G.lucidum spore powder,and its main active ingredients are unsaturated fatty acids and triterpenoid ganoderic acid.However,the unsaturated fatty acids in it are easily oxidized and volatile,so researchers have added vitamin E to solve the problem.At present,no systematic study on the antioxidant stress activity of G.lucidum spore oil supplemented with vitamin E has been reported.Therefore,two samples,G.lucidum spore oil(GLSO)and G.lucidum spore oil with 5%vitamin E(GLSO+VE),were selected to evaluate and compare their protective effects against oxidative stress damage on H2O2-treated rat renal tubular epithelial NRK-52E cells.The experimental results showed that GLSO and GLSO+VE increased cell viability,increased the activity of antioxidant enzymes,and decreased oxidative products with statistical significance.The addition of vitamin E could significantly reduce the cytotoxic effect of G.lucidum spore oil and increase its antioxidant activity to a certain extent.This study preliminarily confirms the enhanced effect of adding vitamin E to G.lucidum spore oil in combating cellular oxidative damage,and provides more theoretical basis and supporting data for research on increasing the stability of G.lucidum spore oil by adding vitamin E.

关 键 词：灵芝孢子油 维生素E 氧化损伤 NRK-52E细胞 

分 类 号：S646.9[农业科学—蔬菜学]