Target-induced Trivalent G-quadruplex/hemin DNAzyme for Colorimetric Detection of Hg^(2+) Based on an Exonuclease III Assisted Catalytic Hairpin Assembly  

作　　者：Zhenghua LIU Zhonghai LI 

机构地区：[1]College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha,410004,China [2]Technology Center of Changsha Customs District of People s Republic of China,Changsha,410004,China

出　　处：《Agricultural Biotechnology》2024年第1期51-57,共7页农业生物技术（英文版）

基　　金：Supported by The Science and Technology Project of General Administration of Quality Supervision,Inspection and Quarantine (2015IK126);The Science and Technology Project of Changsha City of Hunan Province of China (KQ1602124).

摘　　要：Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal output.The developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)detection.Such a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal ions.This strategy shows the great potential for visual detection with portable type.

关 键 词：G-quadruplex/hemin DNAzyme Multivalence Catalytic hairpin assembly Exonuclease III Signal amplification Colorimetric detection 

分 类 号：O64[理学—物理化学]