基于谱效关系的昆布自由基清除活性成分研究  

Radical-Scavenging Active in Kunbu(Laminariae Thallus)Based on Spectrum-Effect Relationship

作　　者：黄华花 郑姗姗 许燕瑜 郑淑文 陈泓旭 HUANG Huahua;ZHENG Shanshan;XU Yanyu;ZHENG Shuwen;CHENG Hongxu(Department of Pharmacy,Xiamen Medical College/Xiamen Key Laboratory of Traditional Chinese Medicine Bioengineering/Key Laboratory of Traditional Chinese Medicine Finish Processing and Health Product,Xiamen Fujian 361023,China)

机构地区：[1]厦门医学院药学系/厦门市中药生物工程重点实验室/福建省中药精加工与健康产品开发重点研究室,福建厦门361023

出　　处：《中医药导报》2024年第2期48-52,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：厦门医学院校级课题(K2022-09);福建省大学生创新创业训练计划项目(202212631020)。

摘　　要：目的:在现有昆布指纹图谱研究的基础上,探讨昆布自由基清除活性的谱效关系,筛选与自由基清除活性密切相关的组分。方法:通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除法测定11批昆布样品的自由基清除活性,结合指纹图谱研究,利用灰色关联度法(GRA)和偏最小二乘回归分析法(PLSR)分析相关成分,建立昆布自由基清除活性的谱效关系。结果:11批样品清除DPPH自由基的IC50为0.0392~0.1044 g/mL,清除ABTS自由基的IC50为0.0022~0.0116 g/mL。灰色关联度分析表明,清除DPPH自由基能力关联度大于0.8的特征峰有X24、X21、X22、X4、X27、X28、X23、X29、X9、X18、X17、X12、X25、X20、X10,清除ABTS自由基能力关联度大于0.8的特征峰有X18、X22、X9、X4、X24、X12、X17、X27;偏最小二乘回归分析表明,清除DPPH自由基能力VIP>1的特征峰有X28、X24、X27、X29、X30、X8、X18,清除ABTS自由基能力VIP>1的特征峰有X30、X25、X18、X24、X33、X17。上述分析结果的共有峰为X18、X24。结论:X18、X24所代表的成分可能是昆布自由基清除活性的药效物质基础。Objective:To establish the spectrum-effect relationship of the radical-scavenging activity in Kunbu(Laminariae thallus),and screen out the components closely related to this effect,on the basis of the previous fingerprint study of Kunbu(Laminariae thallus).Methods:The radical-scavenging activities of 11 batches of Kunbu(Laminariae thallus)were determined by 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical.Based on previous UPLC fingerprint study,grey relation analysis(GRA)and partial least squares regression analysis(PLSR)were adopted to detect the spectrum-effect relationship of the radical-scavenging activity.Results:The 11 batches of Kunbu(Laminariae thallus)have an IC50 of 0.0392-0.1044 g/mL for the scavenging of DPPH radical,and 0.0022-0.0116 g/mL for scavenging of ABTS radical.Additionally,GRA showed that the characteristic peaks with a correlation degree greater than 0.8 for DPPH radical scavenging ability include X24,X21,X22,X4,X27,X28,X23,X29,X9,X18,X17,X12,X25,X20 and X10,and that the characteristic peaks with a correlation degree greater than 0.8 in the ability to clear ABTS free radicals include X18,X22,X9,X4,X24,X12,X17 and X27.PLSR showed that the characteristic peaks with a DPPH free radical scavenging ability VIP>1,include X28,X24,X27,X29,X30,X8 and X18,and that the characteristic peaks with ABTS free radical scavenging ability VIP>1,include X30,X25,X18,X24,X33 and X17.The common peaks were X18 and X24 in the above analysis results.Conclusion:The components represented by X18 and X24 may be the pharmacodynamic basis of the radical-scavenging activity of Kunbu(Laminariae thallus).

关 键 词：昆布 自由基清除活性 谱效关系 灰色关联度分析法 偏最小二乘回归分析法 

分 类 号：R284.1[医药卫生—中药学]