硫酸盐还原菌的生长因子分析及脱硫性能研究  

Growth factor analysis and desulfurization performance of sulfate-reducing bacteria

作　　者：周天然 狄军贞[1] ZHOU Tianran;DI Junzhen(College of Civil Engineering,Liaoning Technical University,Fuxin 123000,China)

机构地区：[1]辽宁工程技术大学土木工程学院,辽宁阜新123000

出　　处：《工业水处理》2024年第3期120-126,共7页Industrial Water Treatment

基　　金：国家自然科学基金项目(41672247,41102157);辽宁省“兴辽英才”青年拔尖人才计划支持项目(XLYC1807159);辽宁省教育厅重点项目(LJKZ0324);辽宁工程技术大学校“双一流”创新团队项目(LNTU20TD-21)。

摘　　要：硫酸盐还原菌(SRB)是一类利用有机物还原SO_(4)^(2-)产生硫化物的细菌。SRB可用于处理含SO_(4)^(2-)废水,但是脱硫效果受温度、环境pH、S^(2-)质量浓度、m(COD)/m(SO_(4)^(2-))等因素限制。基于此,本研究采用批量实验,综合分析温度、环境pH、S^(2-)质量浓度和m(COD)/m(SO_(4)^(2-))这4个影响因素对SRB生长的影响,并探究SRB在不同环境下的脱硫性能。结果表明:培养14~86 h时,SRB处于对数期,此时其活性最高;SRB的最佳生长温度为35℃;体系中的S^(2-)会影响SRB生长,当S^(2-)质量浓度增加时,不仅会抑制SRB的代谢活性,甚至可导致SRB细胞凋亡;SRB能在环境pH为5~8的条件下存活,当pH为7~8时,SRB的代谢最旺盛;最适宜SRB生长的m(COD)/m(SO_(4)^(2-))为2。在最佳条件,即温度35℃、pH=7、m(COD)/m(SO_(4)^(2-))=2条件下,采用Starkey培养基培养处于对数期的SRB 8 d后,溶液OD600、pH、ORP、电导率(EC)、SO_(4)^(2-)去除率分别为1.33、8.78、-393 mV、2.90 mS/cm、84.77%。Sulfate-reducing bacteria(SRB)was a class of bacteria that use organic matter to reduce SO_(4)^(2-)to produce sulfides.SRB can be used to treat wastewater containing SO_(4)^(2-),but the desulfurization effect is limited by temperature,environmental pH,S^(2-)concentration,m(COD)/m(SO_(4)^(2-))and other factors.Based on this,this study used batch experiments to comprehensively analyze the effects of temperature,environmental pH,S^(2-)mass concentration and m(COD)/m(SO_(4)^(2-))on the growth of SRB,and explored the desulfurization performance of SRB in different environments.The results showed that SRB was in the logarithmic phase when cultured for 14-86 h,and the activity was the highest.The optimum growth temperature of SRB was 35℃.The S^(2-)in the system would affect the growth of SRB.When the mass concentration of S^(2-)increased,it would not only inhibit the metabolic activity of SRB,but also lead to apoptosis of SRB cells.SRB could survive under the condition of pH 5-8.When the pH of the environment was 7-8,the metabolism of SRB was the most vigorous.The optimum m(COD)/m(SO_(4)^(2-))for SRB growth was 2.Under the optimal conditions of temperature 35℃,pH=7,and m(COD)/m(SO_(4)^(2-))=2,SRB in logarithmic phase was cultured on Starkey medium for 8 days,the OD600,pH,ORP,EC,and SO_(4)^(2-)removal rates of the solution were 1.33,8.78,-393 mV,2.90 mS/cm,and 84.77%,respectively.

关 键 词：硫酸盐还原菌 酸性矿山废水 生长因子 脱硫性能 

分 类 号：X703[环境科学与工程—环境工程]