基于粘度的现场快速检测方法及应用  

On-site rapid detection methods based on viscosity and their applications

作　　者：张浩 刘书花 毕研慧 赵梅 武文丽 胡琼政 ZHANG Hao;LIU Shuhua;BI Yanhui;ZHAO Mei;WU Wenli;HU Qiongzheng(College of Chemistry and Pharmacy,Qilu University of Technology(Shandong Academy of Sciences),Shandong Analysis and Test Center,Jinan 250014,China)

机构地区：[1]齐鲁工业大学(山东省科学院)化学与制药学部,山东省分析测试中心,济南250014

出　　处：《分析试验室》2024年第2期195-207,共13页Chinese Journal of Analysis Laboratory

基　　金：山东省自然科学基金(ZR2022YQ12,ZR2022MB147);山东省科技型中小企业创新能力提升工程(2021TSGC1173,2022TSGC2491);泰山学者计划(tsqn201812088);齐鲁工业大学科教产融合试点培优项目(2023PY058)资助。

摘　　要：即时检测技术(POCT)是一种价格低廉、响应灵敏、无需大型仪器、可快速得到检测结果的检测手段,通常由具有便携式“样品进-信号出”功能的生物传感器完成。虽然基于刺激响应聚合物溶胶-凝胶转变构建的生物传感器已有大量的研究报道,开发制备简单、检测快速、灵敏度高、选择性好的生物传感器依然具有十分重要的意义。近年来,研究者开发了基于聚合物粘度变化的新型生物传感器,可以一定程度上克服传统水凝胶传感器的缺点,具有较大的发展潜力。本文主要从检测平台以及输出信号模式两个方面,总结归纳了基于粘度的现场快速检测方法及应用。最后,对其发展趋势进行简要的讨论,以期为基于粘度的POCT技术的后续研究和发展提供参考。Point-of-care testing(POCT) has attracted much attention recently with the merits of low cost, sensitive response, no need for large equipment, and fast data acquisition. POCT is usually achieved with portable "sample input-signal output" biosensors. Although a large number of POCT biosensors based on sol-gel transition of stimuli-responsive polymers have been reported, it is still essential to develop new biosensors with simple preparation, rapid response, high sensitivity, and remarkable sensitivity. Recently, series of biosensors based on the change of polymer viscosity have been emerging, which can largely overcome the shortcomings of traditional hydrogel-based biosensors. In this paper, on-site rapid detection methods based on viscosity and their applications are systematically summarized from two aspects including detection platforms and output signal modes. The development trend of viscosity-based POCT technology is also briefly discussed to provide a reference for the follow-up research.

关 键 词：即时检测技术(POCT) 生物传感器 刺激响应聚合物 粘度 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]