低温胁迫影响大麦小孢子全能性机制解析  

Transcriptomic analysis reveals the effect of cold stress on the totipotency of barley microspores

作　　者：李文瑞 李颖波[1] 郭桂梅[1] 宗营杰 周龙华 徐红卫[1] 刘成洪[1] LI Wenrui;LI Yingbo;GUO Guimei;ZONG Yingjie;ZHOU Longhua;XU Hongwei;LIU Chenghong(Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China;College of Life Sciences,Shanghai Normal University,Shanghai 201400,China)

机构地区：[1]上海市农业科学院生物技术研究所,上海市农业遗传育种重点实验室,上海201106 [2]上海师范大学生命科学学院,上海201400

出　　处：《上海农业学报》2024年第1期7-16,共10页Acta Agriculturae Shanghai

基　　金：国家现代农业产业技术体系建设专项(CARS-05-01A-02);上海市农业科学院卓越团队计划[2022(018)];西藏自治区重大科技专项(XZ202101ZD0004N-02)。

摘　　要：为研究低温对小孢子细胞全能性获得的影响,对低温处理0 d、3 d、7 d的大麦小孢子进行体外培养。结果表明:愈伤产量随着低温处理时间增加而显著增长。转录组分析显示,以低温处理0 d为对照,处理3 d和7 d的差异表达基因分别为5290、7103个。对差异表达基因的GO富集分析显示,在处理3 d和7 d显著富集的生物学过程均为碳水化合物代谢过程、赤霉素分解代谢过程、孢粉素生物合成过程等,细胞组分富集种类均为核小体、类囊体、细胞壁等,分子功能富集种类均为蛋白质异源二聚化活性、DNA结合转录因子活性等。KEGG富集分析显示,低温处理3 d和7 d中的通路富集为光合作用-天线蛋白、植物激素信号转导、乙醛酸和二羧酸代谢等。植物激素信号转导通路在上调富集的通路中最显著,相关的基因可能在低温诱导小孢子全能性中起到重要作用。另外,与植物防御机制相关的热休克蛋白和谷胱甘肽转移酶基因也可能参与小孢子重编程过程。To investigate the effects of low temperature on the pluripotency of microspore cells,barley microspores treated with low temperature for 0,3,and 7 days were cultured in vitro.The results showed that the yield of MDEC increased significantly depending upon cold treatment time.Transcriptome analysis showed that 5290 and 7103 differentially expressed genes were obtained at 3 days post treatment(dpt)and 7 dpt when compared with 0 dpt,respectively.GO enrichment analysis showed that the most significant categories in the biological processes at 3 dpt and 7 dpt were carbohydrate metabolism,glutathione metabolic,and sporopollenin biosynthetic process.Nucleosomes,thylakoids,cell wall and other cellular components,as well as DNA binding transcription factor activity were most significant categories in the molecular function.KEGG enrichment analysis showed that the pathways at 3 dpt and 7 dpt were enriched in photosynthesis-antenna proteins,hormone signal transduction,glyoxylate and dicarboxylate metabolism,etc.Plant hormone signal transduction pathway was the most significant among the up-regulated pathways,and the related genes might play important roles in the microspore totipotency.In addition,heat shock protein and glutathione S-transferase,which were related to plant defense pathway,might also be involved in the process of microspore reprogramming.

关 键 词：大麦 小孢子 低温胁迫 转录组分析 

分 类 号：S512.3[农业科学—作物学]