单宁酸对豌豆蚜谷胱甘肽-S-转移酶活性与基因表达的影响  

Effect of tannic acid on glutathione-S-transferase activity and gene expression in Acyrthosiphon pisum

作　　者：王继祖 刘磊 王森山 宋丽雯 WANG Jizu;LIU Lei;WANG Senshan;SONG Liwen(College of plant propection,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃兰州730070

出　　处：《草业科学》2024年第2期459-467,共9页Pratacultural Science

基　　金：甘肃省高等学校创新基金项目(2022A-060);甘肃农业大学公招博士科研启动项目(GAN-KYQD-2019-28)。

摘　　要：本研究通过测定单宁酸(2 g·L^(-1))处理豌豆蚜(Acyrthosiphon pisum) 48 h后体内谷胱甘肽-S-转移酶(GST)活性以及不同组织GST基因转录水平的变化,以明确单宁酸对GST活性以及基因表达的影响。结果表明:豌豆蚜取食单宁酸(2 g·L^(-1)) 48 h后,GST活性下降。相比不处理对照,经单宁酸处理后,豌豆蚜头部有7个GST基因上调表达,其中ApGST104、ApGSTX1上调表达3倍左右;胸部有10个GST基因上调表达,其中ApGSTD6上调表达6.4倍;腹部有10个GST基因上调表达,其中ApGST101、ApGST105上调5倍左右;中肠有9个GST基因上调表达,其中ApPGST105上调表达3.2倍。从结果可以看出,单宁酸对豌豆蚜GST基因的表达产生了影响,部分GST基因显著上调表达(P <0.05),表明GST基因可能在豌豆蚜对单宁酸的代谢中发挥重要的作用。In this study,we aimed to determine the effects of tannic acid(2 g·L^(-1))on glutathione-S-transferase(GST)activity and gene expression in pea aphids(Acyrthosiphon pisum).Specifically,we measured the changes in GST activity and GST gene expression in different tissues of pea aphids after 48 hours of tannic acid treatment.The results show that GST activity decreased following tannic acid(2 g·L^(-1))treatment.Conversely,GST gene expression was affected;compared to the control,seven GST genes were up-regulated in the head of pea aphids,of which ApGST104 and ApGSTX1 were upregulated approximately threefold.Moreover,ten GST genes were upregulated in the chest,of which ApGSTD6 was upregulated 6.4 times higher than the control.Ten GST genes were upregulated in the abdomen,of which ApGST101 and ApGST105 were upregulated approximately fivefold.Nine GST genes were upregulated in the midgut,of which ApGST105 was upregulated by 3.2 times.These results show that tannic acid affected GST gene expression in pea aphids,with some GST genes being significantly upregulated(P<0.05).This result indicates that GST genes may play an important role in the metabolism of tannic acid in pea aphids.

关 键 词：豌豆蚜 解毒酶 植物次生物质 中肠 GST基因 组织解剖 实时荧光定量PCR 

分 类 号：S433[农业科学—农业昆虫与害虫防治]