高寒地区菊芋青贮物料耐低温乳酸菌的筛选与鉴定  

Identification of low-temperature-tolerant lactic acid bacteria in Helianthus tuberosus silage in alpine regions

作　　者：魏晓强 孙雪梅[1,2] 张海旺 王丽慧 杨世鹏[1,2] 司诚 钟启文[1,2] 芦光新 WEI Xiaoqiang;SUN Xuemei;ZHANG Haiwang;WANG Lihui;YANG Shipeng;SI Cheng;ZHONG Qiwen;LU Guangxin(Qinghai Academy of Agricultural and Forestry Sciences,Xining 810016,Qinghai,China;Laboratory for Research and Rtilization of Germplasm Resources in Qinghai Tibet Plateau,Xining 810016,Qinghai,China;College of agriculture and animal husbandry,Qinghai University,Xining 810016,Qinghai,China)

机构地区：[1]青海大学农林科学院,青海西宁810016 [2]青藏高原种质资源研究与利用实验室,青海西宁810016 [3]青海大学农牧学院,青海西宁810016

出　　处：《草业科学》2024年第2期480-490,共11页Pratacultural Science

基　　金：青海省重点研发项目(2022-NK-122);青海省自然科学基金项目(2022-ZJ-902);国家大宗蔬菜产业技术体系项目(CARS-23-G26)。

摘　　要：为筛选可提升高寒地区菊芋(Helianthus tuberosus)青贮发酵品质的耐低温乳酸菌,本研究以‘青芋2号’菊芋青贮物料为材料,利用平板培养法分离乳酸菌株,分析菌株生理生化特性,并通过16S rRNA测序进行菌株种属鉴定。结果表明,菊芋青贮物料中共分离得到34株乳酸菌株,经低温及生理生化筛选获得13株耐低温乳酸菌株,包括11株同型发酵乳酸菌和2株异型发酵乳酸菌,其耐酸性和耐盐性均较强;经产酸和生长能力对比,GN02菌株生长最快(OD=2.63),GN10菌株具有更强的产酸能力(pH=3.59),XN25菌株兼具较强的生长和产酸能力;16S rRNA测序鉴定出9株植物乳杆菌(Lactiplantibacillus plantarum)、1株戊糖乳杆菌(Lactiplantibacillus pentosus)、1株棒状乳杆菌(Lactiplantibacillus coryniformis)、1株短乳杆菌(Lactiplantibacillus brevis)及1株无法鉴定。本研究筛选出适合高寒地区菊芋青贮发酵的耐低温植物乳杆菌、戊糖乳杆菌、短乳杆菌各1株。This study aimed to identify low temperature-tolerant lactic acid bacteria(LAB)that can improve the fermentation quality of Helianthus tuberosus silage in alpine regions.The H.tuberosus silage named‘Qingyu No.2’was used as the material,and LAB was separated using the plate method.Physiological and biochemical characteristics were used to analyze the characteristics of the strains,and 16S rRNA sequencing was conducted to identify the strain species.The results showed that a total of 34 LAB were separated.Among them,13 LAB,including 11 homotypic fermented LAB and two heterotypic fermented LAB,were identified with low temperature tolerance by physiological and biochemical screening.It was found that the strain GN02 grew faster(OD=2.63).GN10 was found to have a stronger acid-producing capacity(pH=3.59)by comparing the acid production and growth capacity at low temperatures,and LAB XN25 combined the two characteristics.Nine strains of Lactiplantibacillus plantarum,one strain of L.pentosus,one strain of L.coryniformis,one strain of L.brevis,and one strain that could not be identified were detected through 16S rRNA sequencing at species level.The study found the potential low-temperature-tolerant LAB,one strain of L.plantarum,one strain of L.pentosus,and one strain of L.brevis suitable for the fermentation of H.tuberosus silage in alpine regions.

关 键 词：高寒地区 菊芋 青贮 平板培养法 耐低温乳酸菌 16S rRNA测序 菌株鉴定 

分 类 号：S816.53[农业科学—饲料科学]