经典藏药如意珍宝片多次给药的靶器官-脑组织的成分探究  

Composition Exploration of Target Organs-brain Tissue of Traditional Tibetan Medicine Ruyi Zhenbao Tablet after Repeated Administration

作　　者：侯红平[1] 王彩霞[1] 彭博[1] 刘荣霞 李冰 陈腾飞 李峰 车向宜 宋玲 高云航 罗倩 叶祖光[1] 张广平[1] HOU Hongping;WANG Caixia;PENG Bo;LIU Rongxia;LI Bing;CHEN Tengfei;LI Feng;CHE Xiangyi;SONG Ling;GAO Yunhang;LUO Qian;YE Zuguang;ZHANG Guangping(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Pharmacy,Yantai University,Yantai 264005,China;Yantai Saipute Testsing Service Co.,Ltd,Yantai 264005,China;Gansu Cheezheng Tibetan Medicine Co.,Ltd.,Lanzhou 730100,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]山东烟台大学,烟台264005 [3]烟台赛普特检测服务有限公司,烟台264005 [4]甘肃奇正藏药有限公司,兰州730100

出　　处：《世界中医药》2024年第2期139-146,共8页World Chinese Medicine

基　　金：国家重点研发计划项目(2019YFC1712403)——经典藏药如意珍宝片和白脉软膏治疗藏医重大疾病白脉病的示范开发研究;中央级公益性科研院所基本科研业务费专项基金资助项目(ZZ13-YQ-060,ZXKT21020)——基于PINK1/Parkin介导的线粒体自噬动态平衡在痰热清干预慢阻肺的机制研究。

摘　　要：目的:探讨经典藏药如意珍宝片多次给药后,其靶器官脑组织的活性成分,分析其可能的作用特点。方法:6只斯泼累格·多雷(SD)雄性大鼠分为对照组和观察组2组,连续给药7 d,在最后一次给药后1 h处死动物,取血浆和脑组织,分析入血成分,并对潜在入脑成分进行检测和验证。结果:采用Full mass/dd-MSMS模式,以目标峰的一级谱图的分子量的组成及二级质谱图的分子离子峰进行定性分析;并依据入血成分作为潜在入脑成分,将其输入到包含列表内,进行样品检测。结合数据库mzcloud和ChemSpider的结果,对潜在入脑成分进行搜索,根据保留时间、一级质量数和二级质谱数据,共发现了13个入脑成分,其中大豆苷、胆酸、紫苜蓿酮、3′-O-methylviolanone、胡椒碱已用标准品验证。色氨酸、茴香脑、异木香酸和腺苷已通过mzCloud数据库进行二级碎片信息比对。结论:如意珍宝片中的降香、荜茇、甘草膏、肉桂、木香、黄葵子、牛黄、红花等药物的有效成分能够进入靶器官脑组织中,发挥其疗效作用。Objective:To explore the active components in the target organ-brain tissue of Ruyi Zhenbao tablet,a classic Tibetan medicine,after repeated administration,and analyze its possible action characteristics.Methods:Six male SD rats were divided into a control group and an observation group,and were continuously administered for 7 days.The rats were sacrificed one hour after the last administration,and the blood plasma and brain tissue were collected.The components entering blood were analyzed,and the potential components entering the brain were detected and verified.Results:Full MS/dd-MS/MS model was used to qualitatively analyze the molecular weight composition of the first-order spectra of the target peak and the molecular ion peak of the second-order spectra.According to the components entering blood as the potential components entering the brain,they were input into the included list for sample detection.Combined with the results of databases mzCloud and ChemSpider,the potential components entering brain were searched.According to the retention time,primary mass number and secondary ion mass spectrometry data,a total of 13 components entering brain were found,among which daidzein,cholic acid,alfalfa ketone,3′-O-methylviolanone and piperine have been verified by standard substances.Tryptophan,anethole,isocostic acid and adenosine have been compared for secondary mass spectral fragment information through the mzCloud database.Conclusion:The effective components of drugs such as Dalbergiae Odoriferae Lignum,Piperis Longi Fructus,Licorice paste,Cinnamomi cortex,Aucklandiae radix,Hibiscus abelmoschus seeds,Calculus bovis,Carthami flos in Ruyi Zhenbao tablet can enter the target organ brain tissue and exert their curative effects.

关 键 词：经典藏药 如意珍宝片 多次给药 组织定性 脑组织 靶器官 牛黄 红花 

分 类 号：R29[医药卫生—民族医学]