4种绿潮藻响应高温胁迫的转录组学研究  

Transcriptome on Response of Four Species of Green Tide Algae to Heat Stress

作　　者：赵卉 周玲洁[1] 蔡春尔[1,2] 何培民[1,2] ZHAO Hui;ZHOU Lingjie;CAI Chun′er;HE Peimin(College of Oceanography and Ecological Science,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学海洋科学与生态环境学院,上海201306 [2]上海海洋大学,水产科学国家级实验教学示范中心,上海201306

出　　处：《水产科学》2024年第2期199-214,共16页Fisheries Science

基　　金：上海市自然科学基金资助项目(18ZR1417400).

摘　　要：将2008年7月在青岛沿岸采集的黄海绿潮藻浒苔、扁浒苔、缘管浒苔及曲浒苔经最适条件下纯系培养后留存的子代分为2组(对照组和处理组),对照组在最适温度、处理组在30℃下培养24 h后,取出藻体,经液氮处理后于-80℃保存后直至提取RNA,进行RNA质控检测和转录组高通量测序。试验结果显示,4种浒苔差异基因个数依次为:扁浒苔>曲浒苔>浒苔>缘管浒苔,这些差异基因主要富集在运输和分解代谢、脂质代谢、氨基酸代谢和能量代谢4部分代谢通路上,而浒苔差异基因特有的富集通路是氧化磷酸化、乙醛酸和二羧酸代谢和ABC转运体,这可能与浒苔响应高温胁迫特殊性有关。4种浒苔都具有完整的热休克蛋白合成和抗氧化物体系,扁浒苔热休克蛋白相关基因表达下调可能是其最不适应高温的原因。不同浒苔也会通过不同抗氧化物酶的表达来抵御高温,其中超氧化物歧化酶的作用最重要。试验结果可为进一步研究4种浒苔在绿潮暴发过程中的演替情况提供基础数据。The Yellow Sea green tide has seriously damaged the marine ecology and caused huge economic loss and serious social influence.Four species of green tide algae Ulva prolifera,U.linza,U.flexuosa,and U.compressa were collected from the coast of Qingdao in July 2008,and the progeny remaining after pure line culture under suitable conditions were divided into two groups:the control group was treated at the optimum temperature and the treatment group was treated at 30°C for 24 h.The algae were taken out,treated with liquid nitrogen and stored at-80°C until RNA extraction,RNA quality control detection and transcriptome by high-throughput sequencing.It was found that the descending order of the number of differential genes in four Ulva species was expressed as U.compressa>U.flexuosa>U.prolifera>U.linza.These differential genes were enriched in four metabolic pathways of transport and catabolism,lipid metabolism,amino acid metabolism and energy metabolism,while the differential genes in U.prolifera were enriched in pathways specific to oxidative phosphorylation,glyoxylate and dicarboxylic acid metabolism and ABC transporter,which may be related to the special response of U,prolifera to high temperature stress.The four types of Ulva all had complete heat shock protein synthesis and antioxidant systems,and the down-regulation of heat shock protein-related gene expression in Ulva compressa.Different Ulva resisted high temperature through the expression of different antioxidant enzymes,in which the role of superoxide dismutase was the most important.The finding provides theoretical basis for succession of four Ulva species during the outbreak of green tide.

关 键 词：浒苔 缘管浒苔 扁浒苔 曲浒苔 转录组 高温 

分 类 号：Q945.78[生物学—植物学]