利用模式动物斑马鱼构建中药抗缺氧活性快速评价模型  

Construction of Rapid Evaluation Model for Anti-Hypoxia Activity of Chinese Medicine Using Model Animal Zebrafish

作　　者：马智慧 刘慧茹 丛媛青 韩欣颖 王松松 曾克武 韩利文 李正国 MA Zhihui;LIU Huiru;CONG Yuanqing;HAN Xinying;WANG Songsong;ZENG Kewu;HAN Liwen;LI Zhengguo(College of Pharmacy,Shandong University of Tra-ditional Chinese Medicine,Jinan 250355,China;School of Pharmaceutical Sciences&Institute of Materia Medica,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;Jining Food and Drug Inspection and Testing Research Institute,Jining 272027,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东第一医科大学(山东省医学科学院)药学院(药物研究所),山东济南250117 [3]山东省济宁市食品药品检验检测研究院,山东济宁272027

出　　处：《山东中医药大学学报》2024年第2期210-216,共7页Journal of Shandong University of Traditional Chinese Medicine

基　　金：鲁渝科技协作计划项目(编号:2020LYXZ012,2020LYXZ034);山东第一医科大学学术提升计划项目(编号:2019LJ003)。

摘　　要：目的:利用模式动物斑马鱼构建适用于中药抗缺氧活性成分研究的快速评价模型。方法:实验选用AB品系斑马鱼,以红景天胶囊为阳性药,以亚硫酸钠为低氧剂,以斑马鱼缺氧后的神经运动行为作为评价指标,考察受精后发育至3 d、4 d、5 d的斑马鱼幼鱼给药预处理开始时间和24 h、48 h、72 h药物预保护时间,建立斑马鱼幼鱼缺氧模型;基于优化的预处理时间及预保护时间,分别检测各组斑马鱼缺氧后的平均心率、脑组织细胞凋亡数量,进一步验证缺氧模型。结果:实验选用发育至5 d的斑马鱼,给药预保护时间48 h,以斑马鱼缺氧后不同阶段的神经运动行为为评价指标,建立缺氧模型。进一步模型评价发现,红景天胶囊预保护组明显增强了缺氧斑马鱼的平均心率,同时降低了由缺氧导致的脑组织细胞凋亡数量(P<0.01)。结论:亚硫酸钠造模后通过斑马鱼不同阶段的神经运动行为可快速评价中药样品的抗缺氧活性模型,为中药药效成分的筛选发现提供关键模型。Objective:To construct a rapid evaluation model for the study of anti-hypoxia active components in Chinese medicine using model animal zebrafish.Methods:The AB strain zebrafish were used in the experiment,with Hongjingtian Capsule(红景天胶囊)as the positive drug and sodium bisulfite as the hypoxia agent.Based on the neurobehavioral evaluation indicators after hypoxia in zebrafish,the starting time for drug pretreatment and the 24 h,48 h,and 72 h drug pre-protection time were investigated in zebrafish larvae after fertilization which developed to 3 d,4 d,5 d to establish a zebrafish larval hypoxia model.Based on the optimized pretreatment time and pre-protection time,the average heart rate after hypoxia and the number of brain tissue cell apoptosis in each group of zebrafish were detected to validate the hypoxia model.Results:Zebrafish developed until 5 d were selected for the experiments,with the drug pre-protection time of 48 h,and the hypoxia model was established using neurobehavioral evaluation indicators at different stages after hypoxia in zebrafish.Further model evaluation found that the Hongjingtian Capsule pre-protection group significantly enhanced the average heart rate in zebrafish caused by hypoxia and simultaneously reduced the number of brain tissue cell apoptosis caused by hypoxia(P<0.01).Conclusions:The neurobehavioral evaluation of zebrafish at different stages after sodium bisulfite modelling can rapidly evaluate the anti-hypoxia activity model of Chinese medicine samples,providing a key model for the screening and discovery of pharmacodynamic components of Chinese medicine.

关 键 词：斑马鱼 亚硫酸钠 红景天胶囊 神经运动行为 抗缺氧活性成分 运动速度与距离 脑细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]