耐药结核病实验室诊断技术研究进展  

Research progress on laboratory diagnostic techniques for drug-resistant tuberculosis

作　　者：李宁迪 江渊[1] LI Ningdi;JIANG Yuan(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]上海市疾病预防控制中心,上海200336

出　　处：《检验医学》2024年第2期203-208,共6页Laboratory Medicine

基　　金：上海市加强公共卫生体系建设三年行动计划(2023—2025)重点学科项目(GWVT-11.1-05);上海市加强公共卫生体系建设三年行动计划(GWVI-9)。

摘　　要：结核病(TB)是严重危害公众健康的全球性公共卫生问题。耐药结核病(DR-TB)是TB防治的重点和难点。目前,常用的体外药物敏感性试验方法包括表型药物敏感性检测技术和分子药物敏感性检测技术。表型药物敏感性检测技术包括固体药物敏感性试验、液体药物敏感性试验、最低抑菌浓度法,分子药物敏感性检测技术包括GeneXpert MTB/RIF、线性探针技术、基因芯片技术、荧光聚合酶链反应(PCR)熔解曲线法、FluoroType MTBDR、Xpert MTB/XDR、核酸质谱分析、全基因组测序技术。文章就表型药物敏感性检测和分子药物敏感性检测对DR-TB实验室诊断技术的研究进展进行综述,旨在为DR-TB的早期诊断提供参考。Tuberculosis(TB)is one of the most serious global public health threats at present.Drug-resistant tuberculosis(DR-TB)is the focus and difficulty of TB prevention and treatment.This review focuses on the research progress of laboratory diagnostic techniques for DR-TB in terms of phenotypic drug susceptibility testing,including susceptibility testing for anti-TB agents using solid or liquid media and minimum inhibitory concentration methods.Molecular drug susceptibility testing includes GeneXpert MTB/RIF,line probe assay,gene chip method,fluorescence polymerase chain reaction(PCR)melting curve method,FluoroType MTBDR,Xpert MTB/XDR,nucleic acid mass spectrometry system and whole genome sequencing.The aim of this review is to provide a reference for the early diagnosis of DR-TB.

关 键 词：耐药结核病 表型药物敏感性检测 分子药物敏感性检测 

分 类 号：R52[医药卫生—内科学]