‘贵紫麦1号’生长素内输基因GzLAX3-1B的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of Auxin Input Gene GzLAX3-1B of ‘Guizimai No.1’

作　　者：安畅 李鲁华 杨文荟 熊富敏 王颖茜 任明见[1] 徐如宏[1] An Chang;Li Luhua;Yang Wenhui;Xiong Fumin;Wang Yingxi;Ren Mingjian;Xu Ruhong(Guizhou Subcenter of National Wheat Improvement Center,College of Agriculture,Guizhou University,Guiyang,550025)

机构地区：[1]贵州大学农学院,国家小麦改良中心贵州分中心,贵阳550025

出　　处：《分子植物育种》2024年第6期1759-1766,共8页Molecular Plant Breeding

基　　金：贵州省科技支撑计划项目(黔科合支撑[2021]一般272);贵州省农业成果转化计划项目(黔科合成果[2019]4246号;黔科合平台人才[2017]5788号);贵州省科技计划项目(黔科合基础[2020]1Z018号,黔科合基础[2019]1073号)共同资助。

摘　　要：为了研究小麦生长素内输基因LAX3在小麦生长发育中的功能。本研究利用RT-PCR方法从‘贵紫麦1号’中获取GzLAX3-1B基因cDNA序列全长,并对该基因及其编码蛋白进行生物信息学分析。结果显示:Gz LAX3-1B基因开放阅读框(ORF)为1 587 bp,编码528个氨基酸,具有PLN03074特异性位点,属于SLC5-6-like-sbd超家族。其编码的蛋白含有44个磷酸化位点和10个跨膜结构,是一种无信号肽的不稳定疏水性质膜蛋白。系统进化关系结果表明:‘贵紫麦1号’GzLAX3-1B基因与野生二粒小麦、大麦和油棕的亲缘关系最近。本研究结果为进一步开展小麦LAX3的生物学功能研究提供一定的理论基础和依据。In order to study the function of auxin gene LAX3 in wheat growth and development.In this study,the full-length cDNA sequence of GzLAX3-1B gene was obtained from'Guizimai No.1'by RT-PCR,and the bioinfor-matics analysis of the gene and its encoded protein was carried out.The results showed that the open reading frame(ORF)of GzLAX3-1B gene was 1587 bp,encoding 528 amino acids,with PLN03074 specific site,belonging to SLC5-6-like-sbd superfamily.It encodes a protein with 44 phosphorylation sites and 10 transmembrane structures,which isan unstable hydrophobic plasma membrane protein without signal peptide.The resultsofphylogenetic anal-ysis showed that the GzLAX3-1B gene of'Guizimai No.1'was closely related to wild emmer wheat,barley and oil palm.The results of this study provide a theoretical basis for further study on biological function of wheat LAX.

关 键 词：贵紫麦1号 GzLAX3-1B 基因克隆 生物信息学分析 

分 类 号：S512.1[农业科学—作物学]