银中杨中5条PaMYBs基因克隆及表达特性分析  

Gene Cloning and Expression Characterization of Five PaMYBs in Populus albaxP.berolinensis

作　　者：张潇月[1] 袁启航 郑雨 姜静[1] 刘桂丰[1] Zhang Xiaoyue;Yuan Qihang;Zheng Yu;Jiang Jing;Liu Guifeng(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,150040)

机构地区：[1]东北林业大学,林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《分子植物育种》2024年第6期1850-1860,共11页Molecular Plant Breeding

基　　金：中央高校基本科研业务费专项(2572019DA03)资助。

摘　　要：MYB转录因子是植物中重要的转录因子之一,不仅参与体类黄酮、花青素等合成过程,同时在植物抵御非生物胁迫过程中也起重要作用。本研究以银中杨叶片和花序为材料克隆获得5条R2R3-MYB转录因子(PaMYB116, PaMYB117, Pa MYB118, PaMYB119, PaMYB120),分析显示,5条PaMYBs的ORF分别为789、777、822、825和750 bp,与拟南芥AtMYB113是同源基因,是与花色苷合成相关的旁系同源物。RT-qPCR表明,PaMYB116在花序中表达量最高,PaMYB118、PaMYB120在茎中表达量最高,PaMYB117则在各组织部位中表达量均较低。在银中杨基因组数据中找到5条PaMYBs启动子序列并分别预测其顺式作用元件,根据预测结果分别进行光和ABA处理银中杨,RT-qPCR结果显示:5条PaMYBs基因均可被光诱导表达,其中PaMYB116、PaMYB117、PaMYB118、PaMYB119基因暗处理3 d后进行光照射,1 h后上述基因表达量迅速增加,而PaMYB120则不同,光照48 h时该基因的相对表达量最高;而喷施200μmol/L ABA处理下5条PaMYBs基因的相对表达量均降低。研究结果为揭示银中杨PaMYB基因提供参考。MYB transcription factor is one of the important transcription factors in plants.It not only participates in the synthesis of flavonoids and anthocyanins,but also plays an important role in the process of abiotic stress resistance.In this study,five R2R3-MYB transcription factors(PaMYB116,PaMYB117,PaMYB118,PaMYB119,Pa-MYB120)were cloned from leaves and inflorescence of Populus alba.Analysis showed that the ORFs of the five PaMYBs were 789,777,822,825 and 750 bp,respectively.They were homologous to Arabidopsis AtMYB113 and parahomologous to anthocyanin synthesis.RT-qPCR showed that the expression of PaMYB116 was the highest in inflorescence,PaMYB118 and PaMYB120 were the highest in stems,and PaMYB117 was the lowest in all tissues.Five PaMYBs promoter sequences were found in the genome data of Populus alba and their cis-acting elements were predicted respectively.According to the predicted results,the Populus alba was treated with light and ABA respectively.The results of RT-qPCR showed that the five PaMYBs genes could be induced to express by light,among which PaMYB116,PaMYB117,PaMYB118 and PaMYB119 genes increased rapidly after dark treated for 3 days and then exposed to light for an hour.The relative expression of PaMYB120 was the highest at 48 h of light exposure;the relative expression levels of 5 PaMYBs genes were decreased under 200μmol/L ABA treatment.The results provide a reference for revealing the MYB gene of Populus alba.

关 键 词：银中杨 R2R3-MYB 表达特性 非生物胁迫 

分 类 号：S792.11[农业科学—林木遗传育种]