Biofabrication of nanocomposite-based scaffolds containing human bone extracellularmatrix for the differentiation of skeletal stem and progenitor cells  

作　　者：Yang-Hee Kim Janos M.Kanczler Stuart Lanham Andrew Rawlings Marta Roldo Gianluca Tozzi Jonathan I.Dawson Gianluca Cidonio Richard O.C.Oreffo 

机构地区：[1]Faculty of Medicine,Bone and Joint Research Group,Centre for Human Development,Stem Cells and Regeneration,Institute of Developmental Sciences,University of Southampton,Southampton SO166YD,UK [2]School of Pharmacy and Biomedical Science,University of Portsmouth,Portsmouth PO12DT,UK [3]School of Engineering,Faculty of Engineering and Science,University of Greenwich,Greenwich ME44TB,UK [4]Center for Life Nano-and Neuro-Science(CLN2S),Italian Institute of Technology,00161 Rome,Italy

出　　处：《Bio-Design and Manufacturing》2024年第2期121-136,共16页生物设计与制造（英文）

基　　金：supported by grants from the Biotechnology and Biological Sciences Research Council(Nos.BBSRC LO21071/and BB/L00609X/1);UK Regenerative Medicine Platform Hub Acellular Approaches for Therapeutic Delivery(No.MR/K026682/1);Acellular Hub,SMART Materials 3D Architecture(No.MR/R015651/1);the UK Regenerative Medicine Platform(No.MR/L012626/1 Southampton Imaging)to ROCO;MRCAMED Regenerative Medicine and Stem Cell Research Initiative(No.MR/V00543X/1)to JID,ROCO and YHK;GC acknowledges funding from AIRC Aldi Fellowship under grant agreement No.25412.

摘　　要：Autograft or metal implants are routinely used in skeletal repair.However,they fail to provide long-term clinical resolution,necessitating a functional biomimetic tissue engineering alternative.The use of native human bone tissue for synthesizing a biomimeticmaterial inkfor three-dimensional(3D)bioprintingof skeletal tissueis anattractivestrategyfor tissueregeneration.Thus,human bone extracellular matrix(bone-ECM)offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells(HBMSCs)to proliferate and differentiate along the osteogenic lineage.In this study,we engineered a novel material ink(LAB)by blending human bone-ECM(B)with nanoclay(L,Laponite®)and alginate(A)polymers using extrusion-based deposition.The inclusion of the nanofiller and polymeric material increased the rheology,printability,and drug retention properties and,critically,the preservation of HBMSCs viability upon printing.The composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor(VEGF)enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane(CAM)model.The inclusion of bone morphogenetic protein-2(BMP-2)with the HBMSCs further enhanced vascularization and mineralization after only seven days.This study demonstrates the synergistic combination of nanoclay with biomimetic materials(alginate and bone-ECM)to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair.

关 键 词：Extracellular matrix NANOCLAY Bone 3D bioprinting 

分 类 号：R318[医药卫生—生物医学工程]