野菊尿苷二磷酸糖基转移酶(UGT)基因家族的全基因组鉴定和表达分析  

Identification and expression of genome of uridine diphosphate glycosyltransferase(UGT)gene family from Chrysanthemum indicum

作　　者：廖家豪 陈诗 刘迪 张景景 刘义飞 LIAO Jia-hao;CHEN Shi;LIU Di;ZHANG Jing-jing;LIU Yi-fei(School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《中国中药杂志》2024年第3期702-716,共15页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(32270231);湖北省中医药管理局中医药科研项目(ZY2023F130)。

摘　　要：尿苷二磷酸糖基转移酶(UGT)在植物体内参与多种次生代谢产物的糖基化修饰,在植物生长发育与次生代谢调节中具有重要作用。该研究基于二倍体野菊Chrysanthemum indicum基因组,利用生物信息学方法对野菊UGT基因家族进行鉴定,并对UGT蛋白的理化性质、亚细胞定位预测、保守基序、系统发育、染色体定位、基因结构以及基因复制事件进行分析,利用转录组与实时荧光定量PCR分析野菊UGT基因在花和叶组织中的表达模式,利用类靶向代谢组分析花和叶中差异代谢物。结果显示,在二倍体野菊基因组中共鉴定出279个UGT基因,系统发育分析显示这些UGT基因分为了8个亚家族,同一亚家族成员在染色体上呈簇状分布,串联重复是野菊UGT基因家族扩张的主要驱动力。结构域分析显示,262个UGT基因具有完整的植物次生代谢信号序列(PSPG box)。顺式作用元件分析表明,光响应元件是UGT基因家族成员启动子区域最普遍存在的元件。花和叶组织的类靶向代谢组分析显示,木犀草素-7-O-葡萄糖苷、山柰酚-7-O-葡萄糖苷等多种黄酮类代谢物在花中具有更高的积累水平。花和叶组织的比较转录组分析显示,差异表达的UGT基因有72个,其中29个基因是在花中上调,43个基因在叶中上调。相关性网络与系统发育分析显示CindChr9G00614970.1、CindChr2G00092510.1、CindChr2G00092490.1可能参与了野菊中7-O-黄酮苷的合成。实时荧光定量PCR分析证实了转录组数据的可靠性。该研究结果有助于了解野菊UGT基因家族的功能,为深入研究野菊黄酮苷合成的分子调控机制提供数据参考和理论依据。Uridine diphosphate glycosyltransferase(UGT)is involved in the glycosylation of a variety of secondary metabolites in plants and plays an important role in plant growth and development and regulation of secondary metabolism.Based on the genome of a diploid Chrysanthemum indicum,the UGT gene family from Ch.indicum was identified by bioinformatics methods,and the physical and chemical properties,subcellular localization prediction,conserved motif,phylogeny,chromosome location,gene structure,and gene replication events of UGT protein were analyzed.Transcriptome and real-time fluorescence quantitative polymerase chain reaction(PCR)were used to analyze the expression pattern of the UGT gene in flowers and leaves of Ch.indicum.Quasi-targeted metabolomics was used to analyze the differential metabolites in flowers and leaves.The results showed that a total of 279 UGT genes were identified in the Ch.indicum genome.Phylogenetic analysis showed that these UGT genes were divided into 8 subfamilies.Members of the same subfamily were distributed in clusters on the chromosomes.Tandem duplications were the main driver of the expansion of the UGT gene family from Ch.indicum.Structural domain analysis showed that 262 UGT genes had complete plant secondary metabolism signal sequences(PSPG box).The analysis of cis-acting elements indicated that light-responsive elements were the most ubiquitous elements in the promoter regions of UGT gene family members.Quasi-targeted metabolome analysis of floral and leaf tissue revealed that most of the flavonoid metabolites,including luteolin-7-O-glucoside and kaempferol-7-O-glucoside,had higher accumulation in flowers.Comparative transcriptome analysis of flower and leaf tissue showed that there were 72 differentially expressed UGT genes,of which 29 genes were up-regulated in flowers,and 43 genes were up-regulated in leaves.Correlation network and phylogenetic analysis showed that CindChr9G00614970.1,CindChr2G00092510.1,and CindChr2G00092490.1 may be involved in the synthesis of 7-O-flavonoid g

关 键 词：野菊 尿苷二磷酸糖基转移酶(UGT) 代谢组分析 转录组分析 

分 类 号：S567.239[农业科学—中草药栽培]