玻璃化冷冻时间对冻胚移植妊娠结局的影响  

The effect of vitrification freezing times on the pregnancy outcome of frozen embryo transfer

作　　者：屈潇潇 哈灵侠[2,3] 杜玉冬 王金娟 QU Xiaoxiao;HA Lingxia;DU Yudong;WANG Jinjuan(Ningxia Medical University,Yinchuan 750004,China;Center for Reproductive Medicine,the General Hospital of Ningxia Medical University,Yinchuan 750004,China;Key Laboratory of Fertility Preservation and Maintenance Ministry of Education,NingxiaMedical University,Yinchuan 750004 China;Department of Reproductive Medicine,Luoyang Maternal and Child Health Hospital,Luoyang,471000,China)

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏医科大学总医院生殖医学中心,宁夏银川750004 [3]宁夏医科大学生育力保持教育部重点实验室,宁夏银川750004 [4]洛阳市妇幼保健院生殖医学研究所,河南洛阳471000

出　　处：《宁夏医学杂志》2024年第2期108-112,共5页Ningxia Medical Journal

基　　金：宁夏科技惠民基金项目(2022KJHM0066)。

摘　　要：目的探讨不同玻璃化冷冻时间对冻胚复苏及移植妊娠结局的影响。方法回顾性分析进行冻胚移植(FET)的550例患者的临床资料,根据胚胎不同玻璃化冷冻时间分为A、B、C、D、E共5组,A组239例,玻璃化冷冻时间≤3个月;B组138例,3个月<玻璃化冷冻时间≤6个月;C组74例,6个月<玻璃化冷冻时间≤1年;D组75例,1年<玻璃化冷冻时间≤5年;E组24例,玻璃化冷冻时间>5年。比较不同冷冻时间胚胎移植的一般临床资料、胚胎复苏结局及临床妊娠结局。结果相较于A、B、C组,D组胚胎存活率、胚胎完好率显著降低(P<0.05);相较于A、B组,E组的胚胎存活率、胚胎完好率及解冻后/冷冻前优质胚胎率均显著降低(P<0.05);E组的胚胎完好率较C组显著降低(P<0.05);E组的解冻后/冷冻前优质胚胎率显著低于D组(P<0.05)。各组间移植优质胚胎后的胚胎种植率、临床妊娠率、活产率、流产率及早产率差异无统计学意义。移植优质卵裂期胚胎后,E组早产率较B组显著增高(P<0.05)。移植优质囊胚后,C组早产率较A组显著增高(P<0.05)。结论在冻胚移植周期中,较长的玻璃化冷冻时间对胚胎的复苏结局存在不利影响,但在移植至少一枚优质冷冻胚胎后,不同冷冻时间对胚胎移植临床妊娠结局并无影响。Objective To explore the effects of different vitrification freezing times on frozen embryo recovery and pregnancy outcomes.Method A retrospective analysis was conducted on the clinical data of 550 patients who met the inclusion criteria and underwent frozen embryo transfer.According to different vitrification freezing times of embryos,they were divided into 5 groups:A,B,C,D and E.Group A was 239 cases,with vitrification freezing time≤3 months;Group B was 138 cases,with 3 months<vitrification freezing time≤6 months;Group C was 74 cases,with 6 months<vitrification freezing time≤1 year;Group D was 75cases,with 1 year<vitrification freezing time≤5 years;Group E was 24 cases,with vitrification freezing time>5 years.The general clinical data,embryo recovery outcomes and clinical pregnancy outcomes of embryo transfer with different vitrification freezing times were compared.Results Compared with groups A,B,and C,the embryo survival rate and embryo integrity rate in group D were significantly reduced(P<0.05).Compared with group A and B,the embryo survival rate,embryo integrity rate,and high-quality embryo rate after thawing/before freezing in group E were significantly reduced(P<0.05).The embryo integrity rate of group E was significantly lower than that of group C(P<0.05).The rate of high-quality embryos after thawing/before freezing in group E was significantly lower than that in group D(P<0.05).There were no statistically significant differences in embryo implantation rate,clinical pregnancy rate,live birth rate,miscarriage rate,and premature birth rate among the groups after transferring high-quality embryos.After transferring high-quality cleavage stage embryos,the premature birth rate in group E was significantly higher than that in group B(P<0.05).After transplantation of high-quality blastocysts,the premature birth rate in group C was significantly higher than that in group A(P<0.05).Conclusion In the frozen embryo transfer cycle,a longer vitrification freezing time has a negative impact on the recovery

关 键 词：玻璃化冷冻 冻胚移植 复苏结局 妊娠结局 

分 类 号：R714.8[医药卫生—妇产科学]