娄底市1例人感染H9N2禽流感病毒流行病学调查与基因序列分析  

Epidemiological survey and genetic sequence analysis of a human case infected with avian influenza A(H9N2)virus in Loudi City

作　　者：杨青廷 黄超洋[2] 余彪 曾紫嫣 杨雪艳 刘曙光 朱攀[1] 周丹 杨纲[1] YANG Qingting;HUANG Chaoyang;YU Biao;ZENG Ziyan;YANG Xueyan;LIU Shuguang;ZHU Pan;ZHOU Dan;YANG Gang(Loudi Municipal Center for Disease Control and Prevention,Loudi,Hunan 417000,China;Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410153,China;Xinhua County Center for Disease Control and Prevention,Xinhua,Hunan 417600,China)

机构地区：[1]娄底市疾病预防控制中心,湖南娄底417000 [2]湖南省疾病预防控制中心,湖南长沙410153 [3]新化县疾病预防控制中心,湖南新化417600

出　　处：《实用预防医学》2024年第2期172-175,共4页Practical Preventive Medicine

基　　金：湖南省卫生健康委课题(D202312069067);微生物分子生物学湖南省重点实验室开放基金(2019FW07)。

摘　　要：目的对娄底市1例人感染甲型禽流感H9N2病毒病例的流行病学调查分析和基因序列分析,为今后禽流感的科学防治提供依据。方法2023年2月10日对湖南省娄底市1例诊断为感染甲型禽流感H9N2病毒患者的密切及次密切接触者和当地活禽市场进行流行病学调查,并采集患者和密切及次密切接触者咽拭子进行基因序列分析。结果除该病例外,与患者密切和次密切接触者核酸检测均为阴性。患者家庭住宅及周边鸡血样和环境样,经检测其禽类甲型流感病毒均为阴性,但周边活禽市场所采集检测到H9阳性。基因序列分析显示:HA基因序列与国内以往获得的人感染H9N2流行株的HA同源性为83.6%~98.5%,最接近ON856627/Fujiansiming/2021(H9N2)Human/HA,同源性为98.5%;NA基因序列与国内以往获得的人感染H9N2流行株的NA同源性为83.3%~95.5%。最接近MT875139/A Szuhou/2019(H9N2)Human/NA,同源性为95.5%。结论患者感染甲型禽流感H9N2病毒与当地活禽市场存在禽类甲型流感病毒密切相关,今后可通过增加样本数量和采样频率来改进常规流感样疾病监测。Objective To conduct an epidemiological survey and genetic sequence analysis on a case of human infection with avian influenza A(H9N2)virus in Loudi City so as to provide a basis for scientific prevention and control of avian influenza in future.Methods On February 10,2023,an epidemiological investigation was conducted among close and secondary close contacts of a patient diagnosed with avian influenza A(H9N2)virus infection as well as the nearby live poultry market in Loudi City,Hunan Province.In addition,the patient’s as well as close and secondary close contacts’throat swab samples were collected for genetic sequence analysis.Results All the subjects detected presented negative nucleic acid testing results except the patient.Testing of chicken blood samples and environmental samples from the patient’s residence and its neighborhood also showed negative results for avian influenza viruses,but H9N2 was detected in samples collected from the nearby live poultry market.Genetic sequence analysis revealed that the hemagglutinin(HA)gene sequence of the strains detected and H9N2 strains previously obtained from human infection in China's Mainland displayed homologies ranging from 83.6%to 98.5%,which were closest with ON856627/Fujiansiming/2021(H9N2)Human/HA,with the homology being 98.5%.The neuraminidase(NA)gene sequence of the strains detected and H9N2 strains previously obtained from human infection in China's Mainland displayed homologies ranging from 83.3%to 95.5%,which were closest with MT875139/A Szuhou/2019(H9N2)Human/NA,with the homology being 95.5%.Conclusion The patient’s infection with avian influenza A(H9N2)virus is closely related to the existence of avian influenza A virus in the local live poultry market.In future,routine surveillance for influenza-like illnesses should be improved by increasing the sample size and sampling frequency.

关 键 词：H9N2禽流感 人感染H9N2 活禽市场 基因序列 血凝素 神经氨酸酶 

分 类 号：R511.7[医药卫生—内科学]