细粒棘球蚴感染对小鼠炎症性肠病的保护机制研究  

Protective mechanism of Echinococcus granulosus infection on inflammatory bowel disease in mice

作　　者：孙畅 包建玲[1] 李军[2] 齐文静[2] 张耀[2] 田梦潇 吴川川[2] 张文宝[2] SUN Chang;BAO Jianling;LI Jun;QI Wenjing;ZHANG Yao;TIAN Mengxiao;WU Chuanchuan;ZHANG Wenbao(Medical Laboratory Center of the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang;Clinical Research Institute of the First Affiliated Hospital of Xinjiang Medical University)

机构地区：[1]新疆医科大学第一附属医院医学检验中心,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院临床研究院

出　　处：《中国病原生物学杂志》2024年第3期280-284,共5页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.81830066);国家自然科学基金项目(No.32072886)。

摘　　要：目的观察细粒棘球蚴感染对葡聚糖硫酸钠(DSS)诱导的小鼠炎症性肠病(IBD)症状的保护作用及可能机制。方法建立继发性细粒棘球蚴感染小鼠模型,再给予DSS诱导IBD症状,解剖后ELISA法检测小鼠血清中囊液抗原水平,并观察腹腔内的包囊;根据每日体重监测、解剖后结肠长度测量及结肠组织病理学评分指标评估细粒棘球蚴感染对炎症性肠病的保护作用;采用流式细胞术CBA法检测模型鼠血清中Th1/Th2/Th17水平,评价细胞因子对炎症性肠病的保护作用。结果小鼠感染细粒棘球蚴后血清HCF抗体阳性率与成囊率均为100%。细粒棘球蚴感染减轻了DSS引起的小鼠体重下降程度;结肠变短程度减小,HE染色检查小鼠结肠组织炎性症状显著改善。CBA检测显示细粒棘球蚴感染小鼠血清IL-4、IL-10、IL-17A、IL-6、IFNγ含量均较正常小鼠显著增加,表现为Th2为主的免疫反应;细粒棘球蚴感染合并IBD模型组小鼠Th2/Th1型细胞因子比率同IBD组相比显著升高。结论细粒棘球蚴感染IBD模型小鼠血清IL-4、IL-10水平升高,Th2高水平状态削弱了DSS引发的Th1反应,表明细粒棘球蚴感染可改善IBD小鼠Th1/Th2/Th17细胞因子平衡,IL-4、IL-10可能参与细粒棘球蚴感染对炎症性肠病的保护。Objective To observe the protective effect of Echinococcus granulosus(E.g)metacestode infection against the symptoms of inflammatory bowel disease(IBD)induced by sodium glucose sulfate(DSS)and the underlying mechanisms.Methods a mouse model of secondary E.g infection was established and IBD was induced by DSS.The contration of cyst fluid antigen in the serum these E.g infected mice was test using ELISA;The protective effect of E.g infection on IBD was evaluated through body weight changing,colon length after dissection and colon histopathological score by H&E staining.The levels of Th1/Th2/Th17 cytokines in the sera of mice were detected by flow cytometry CBA method.Results The results showed that all the infected mice were positive in serum antibodies against HCF and 100%of the infected mice had cysts in the addominal cavity.E.g infection reduced the weight loss caused by DSS(P<0.05);The colon length in the infection group was significantly longer than that in the DSS group(P<0.05);H&E staining showed that the inflammatory index of colon tissues in the infected group was lower than that in the DSS group;CBA showed that E.g infection significantly decreased the serum Thl/Th2 cytokine levels and ratios in IBD mice.Compared with the normal control group,E.g infection significantly increased the serum cytokines IL-4,IL-10,IL-17A,IL-6 and IFN-γ(P<0.05)and the Th2/Th1 cytokine ratio in IBD model group previously infected with E.g metacestode was significantly higher than that in the DSS group.Conclusion E.g infection rebalanced the serum cytokine ratio of Th1/Th2/Th17 in IBD mice.Infection with E.granulosus causes a relative increased IL-4 and IL-10 in IBD model mice,and decreases Th1 response induced by DSS,thereby inhibiting the pathological response of IBD.

关 键 词：细粒棘球绦虫 炎症性肠病 保护机制 Th1和Th2 细胞因子 保护 

分 类 号：R532.32[医药卫生—内科学] R574[医药卫生—临床医学]