全肠外营养液中钾离子浓度检测方法的建立与验证  

Determination of potassium ion in total parenteral nutrition based on ion selective electrode

作　　者：杨彦平 张雯雯 李媛[2] YANG Yanping;ZHANG Wenwen;LI Yuan(Department of Pharmacy,The first Affiliated Hospital of Air Force Military Medical University,Shaanxi Xi'an 710032,China;Department of Pharmacy,Xi′an Children′s Hospital,Shaanxi Xi'an 710003,China)

机构地区：[1]空军军医大学第一附属医院药学部,陕西西安710032 [2]西安市儿童医院药学部,陕西西安710003

出　　处：《中国医院药学杂志》2024年第3期295-297,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:基于离子选择电极法原理建立检测全肠外营养液(TPN)中钾离子浓度的快速定性定量方法并进行验证,以期简化临床TPN中钾离子检测流程,为临床TPN中钾离子浓度分析提供依据。方法:根据TPN标准操作规程制备检测样本,经稀释后采用日立7180全自动生化分析仪以离子选择电极法原理检测全肠外营养液中钾离子含量,对临床常用3种TPN中钾离子进行测定以评价该方法的适用性。结果:该方法钾离子浓度范围为6.71~44.74 mmol·L^(-1)时线性良好呈正相关,r=0.9998。TPN中钾离子极低、低、中、高4种浓度的准确度在98.5%~102.6%范围内,批内及批间精密度均小于10%。结论:本法灵敏准确,简便快速,适用于临床TPN中钾离子浓度的快速定性定量检测,可有效减少工作时间,提高工作效率,降低临床输液相关不良反应发生率。OBJECTIVE To establish and verify a rapid qualitative and quantitative method for detecting potassium ions in total parenteral nutrition solution(TPN)based on the ion selective electrode method.Which can simplify the detection process of potassium ions in clinical TPN,and provide a basis for the analysis of potassium ions in clinical TPN.METHODS The test samples were prepared according to the TPN standard operating procedures,and the potassium ions concentration in TPN solution were detected by the Hitachi 7180 automatic biochemical analyzer.The potassium ions in three commonly used TPNs were mea⁃sured,and evaluated the applicability of this method.RESULTS The concentration range of this method is 6.71~44.74 mmol·L^(-1),r=0.9998.The accuracy of the four concentrations of potassium ions in TPN is in the range of 98.5%~102.6%,preci⁃sion<10%.CONCLUSION This method is suitable for rapid qualitative and quantitative detection of potassium ion concentra⁃tion in TPN,which can improve the efficiency of work,and reduce the adverse reactions related to clinical infusion.

关 键 词：全肠外营养液 钾离子 离子选择电极法 

分 类 号：R977[医药卫生—药品]