番石榴果实酵母cDNA文库构建及评价  

Construction and Evaluation of Yeast cDNA Library of Guava Fruit

作　　者：萧允艺 赵晓梦 于泽浩 刘金丰 XIAO Yunyi;ZHAO Xiaomeng;YU Zehao;LIU Jinfeng(College of Biological and Food Engineering,Guangdong University of Petrochemical Technology,Maoming 525000,China;College of Life and Geographic Sciences,Kashi University,Kashi 844006,China)

机构地区：[1]广东石油化工学院生物与食品工程学院,广东茂名525000 [2]喀什大学生命与地理科学学院,新疆喀什844006

出　　处：《广东石油化工学院学报》2024年第1期50-54,共5页Journal of Guangdong University of Petrochemical Technology

基　　金：广东省普通高校特色创新项目(自然科学)(2023KTSCX090);广东省普通高校重点领域专项(乡村振兴)项目(2020ZDZX1056);广东石油化工学院科研基金人才引进项目(2018rc34)。

摘　　要：酵母文库是研究蛋白互作及转录调控的重要基础,构建番石榴果实酵母cDNA文库,是研究番石榴果实采后成熟复杂分子机制的重要途径。首先提取高质量番石榴mRNA,接着合成双链cDNA文库,再通过Gateway位点特异性重组手段将cDNA文库构建至酵母pGADT7-DEST表达载体上。检测结果显示,次级酵母cDNA文库库容量约为1.56×10^(7)cfu,插入cDNA片段长度为0.85~5.00kb,平均长度大于1.00 kb,且多态性丰富,表明文库质量较好。该酵母cDNA文库的构建为获取与果实成熟关键转录因子和系统性研究果实功能基因转录调控机制奠定了基础。Yeast library is an important basis for studying protein interaction and transcriptional regulation.Constructing yeast cDNA library of guava fruit is an important way to study the complex molecular mechanism of postharvest maturation of guava fruit.Firstly,high quality mRNA of guava was obtained,then double-stranded cDNA library was synthesized,and the cDNA library was constructed into yeast pGADT7-Dest expression vector by Gateway site-specific recombinant method.The results showed that the capacity of the secondary yeast cDNA library was about 1.56×10^(7) cfu,the length of inserted cDNA fragments was 0.85~5.00 kb,the average length was greater than 1.00 kb,and the polymorphism was abundant,indicating that the quality of the library was advanced.The construction of yeast cDNA library laid the experimental foundation for harvesting and fruit maturation key transcription factors and systematically studying the transcriptional regulation mechanism of fruit functional genes.

关 键 词：番石榴 酵母文库 文库构建 转录调控 

分 类 号：Q781[生物学—分子生物学]