湖北省2014—2023年登革病毒包膜蛋白基因特征分析  

Analysis of envelope protein gene characteristics of dengue virus in HubeiProvince from 2014 to 2023

作　　者：彭延[1] 蔡昆[1] 李静[1] 邹文菁[1] PENG Yan;CAI Kun;LI Jing;ZOU Wen-jing(Hubei Center for Disease Control and Prevention,Wuhan,Hubei 430079,China)

机构地区：[1]湖北省疾病预防控制中心,湖北武汉430079

出　　处：《现代预防医学》2024年第5期916-920,共5页Modern Preventive Medicine

基　　金：国家自然科学基金区域创新发展联合基金重点项目(U21A20423)。

摘　　要：目的 分析2014—2023年湖北省登革热感染病例包膜蛋白(envelope,E)基因特征并进行生物学溯源方法 收集疑似登革热病例血清标本,对标本进行核酸检测及血清分型,核酸阳性标本采用RT-PCR扩增E基因并进行测序分型,获得基因序列用MEGA 11.0软件进行基因特征分析。结果 2014—2023年湖北省共检测登革热核酸阳性病例78份,成功获得44份标本的登革病毒(dengue virus, DENV)E基因序列,其中血清Ⅰ型(DENV-Ⅰ)39份,血清Ⅱ型(DENV-Ⅱ)5份;进化分析显示,DENV-Ⅰ中37例为基因Ⅰ型(GⅠ),分布在三个不同分支上,主要与中国广州、新加坡、印度尼西亚、泰国和缅甸等东南亚国家流行株进化距离较近,2例为基因Ⅴ型(GⅤ)与中国广州和印度流行株进化距离较近;DENV-Ⅱ中5例均为混合型(cosmopolitan型)与输入地流行株进化距离较近。结论 2014—2023年湖北省存在多型登革病毒感染,主要流行株为DENV-Ⅰ的GⅠ亚型,与中国广州和东南亚国家亲缘关系较近,提示湖北省应加强登革热跨省、跨境传播的防控,同时谨防登革重症及死亡病例的出现。Objective To analyze the characteristics of envelope protein(envelope,E)gene in dengue fever cases in Hubei Province from 2014 to 2023 and to trace the biological origin.Methods Serum samples of suspected dengue fever cases were collected,and nucleic acid detection and serum typing were carried out.Nucleic acid positive samples were amplified by RT-PCR and sequenced,and the gene sequences were obtained and analyzed by MEGA11.0 software.Results A total of 78 dengue nucleic acid positive cases were detected in Hubei Province from 2014 to 2023,and the E gene sequence of dengue virus(DENV)was successfully obtained in 44 samples,including 39 cases of serum typeⅠ(DENV-Ⅰ)and 5 cases of serum typeⅡ(DENV-Ⅱ).Evolutionary analysis showed that 37 cases of DENV-Ⅰwere of gene typeⅠ(GⅠ),distributed in three different branches,mainly close to the epidemic strains of Southeast Asian countries such as Guangzhou,Singapore,Indonesia,Thailand,and Myanmar,2 cases were of gene typeⅤ(GⅤ)were close to the epidemic strains of Guangzhou and India,and 5 cases of DENV-Ⅱwere of mixed type(Cosmopolitan type).Conclusion There is multitype dengue virus infection in Hubei Province from 2014 to 2023.The main epidemic strain is GⅠsubtype of DENV-Ⅰ,which is closely related to Guangzhou,China,and Southeast Asian countries.It is suggested that Hubei Province strengthen the prevention and control of cross-provincial and cross-border transmission of dengue fever and guard against the occurrence of severe dengue and death cases.

关 键 词：登革病毒 包膜蛋白基因 序列分析 

分 类 号：R373.33[医药卫生—病原生物学]