黄芩素对人乳腺癌细胞系MDA-MB-231侵袭、迁移、上皮间充质转化的调控作用及其机制  

Regulatory effects of baicalein on invasion,migration,and EMT in human breast cancer cell line MDA-MB-231

作　　者：陈林[1,2] 梁秋果 吉杨丹 王恒[1,2] CHEN Lin;LIANG Qiuguo;JI Yangdan;WANG Heng(不详;Department of Basic Medicine,Qiannan Medical College for Nationalities,Duyun 558013,China)

机构地区：[1]黔南民族医学高等专科学校基础医学部,贵州都匀558013 [2]黔南州天然产物与组分功效重点实验室

出　　处：《山东医药》2024年第6期10-13,共4页Shandong Medical Journal

基　　金：黔南民族医学高等专科学校校基金(qnyz202013,qnyz202206);黔南民族医学高等专科学教育教学科研基金项目(qnyzjx202205);黔南民族医学高等专科学校大学生科技创新项目(qnyz202201)。

摘　　要：目的观察黄岑素对人乳腺癌细胞系MDA-MB-231的侵袭、迁移及上皮间充质转化(EMT)的调控作用,探讨其可能作用机制。方法取对数生长期MDA-MB-231细胞分为一组、二组、三组及对照组,一组、二组、三组分别加入2.5、5、10μmol/L的黄岑素,对照组不做任何处理。培养48 h时采用划痕修复实验观察四组细胞迁移能力、采用Transwell侵袭实验观察四组细胞侵袭能力,采用Western Blotting法检测细胞EMT标志物波形蛋白(vimentin)及E-钙黏蛋白(E-cadherin)、整合素αv、β3、磷酸化黏着斑激酶(p-FAK)、磷酸化磷脂酰肌醇3激酶(整合素p-PI3K)。结果与对照组相比,黄岑素组细胞迁移率降低、侵袭细胞数少,细胞E-cadherin相对表达量高,vimentin、整合素αv、整合素β3、p-FAK、p-PI3K蛋白相对表达量低,且呈剂量依赖性(P均<0.05)。结论黄芩素抑制MDA-MB-231细胞的侵袭、迁移及EMT。黄岑素可能通过抑制整合素αv、整合素β3表达,进一步抑制p-FAK、p-PI3K蛋白表达,抑制MDA-MB-231的侵袭、迁移及EMT。Objective To investigate the regulatory effects of baicalein on the invasion,migration,and epithelial-mesenchymal transition(EMT)of human breast cancer cell line MDA-MB-231 and to explore its potential mechanism of action.Methods MDA-MB-231 cells in the logarithmic growth phase were divided into the Group 1,Group 2,Group 3,and Control group.Baicalein was added to cells in the Group 1,Group 2,and Group 3 at concentrations of 2.5,5,and 10μmol/L,respectively,while cells in the Control group were not treated.After 48 h of incubation,Scratch repair experi‐ment was used to observe cell migration capacity,Transwell invasion experiment was performed to assess cell invasion ca‐pacity,and Western blotting was utilized to detect the expression levels of EMT markers including vimentin and E-cad‐herin,as well as integrinαv,integrinβ3,phosphorylated focal adhesion kinase(p-FAK),and phosphorylated phosphati‐dylinositol-3 kinase(p-PI3K)proteins.Results Compared with the control group,the cell migration rate decreased,the number of invading cells decreased,the relative expression level of E-cadherin was higher,and the relative expression levels of vimentin,integrinαv,integrinβ3,p-FAK,and p-PI3K protein were lower in the baicalein group,with a dose-de‐pendant manner(all P<0.05).Conclusions Baicalein inhibits the invasion,migration,and EMT of MDA-MB-231 cells.Baicalein may inhibit the expression of integrinαv,integrinβ3,and further inhibit the expression of p-FAK and p-PI3K proteins,thereby inhibiting the invasion,migration,and EMT of MDA-MB-231 cells.

关 键 词：黄芩素 乳腺癌 细胞侵袭 细胞迁移 上皮间质转化 波形蛋白 E-钙黏蛋白 整合素αv、整合素β3 黏着斑激酶 磷脂酰肌醇3激酶 

分 类 号：R965.1[医药卫生—药理学]