2型糖尿病足患者肠道菌群特征及与临床生化指标相关性研究  

A Study on the Distribution Characteristics of Intestinal Flora in Type 2 Diabetic Foot Patients and its Correlation

作　　者：周文志 张莉[2] 王文燕 史雅静 张晶晶 郭丽梅 赵跃斌 ZHOU Wen-zhi;ZHANG Li;WANG Wen-yan;SHI Ya-jing;ZHANG Jing-jing;GUO Li-mei;ZHAO Yue-bin(The Ninth Clinical Medical College of Shanxi Medical University,Taiyuan,Shanxi,030000,China;Endocrine Department of Taiyuan Central Hospital,Taiyuan,Shanxi,030000,China;Endocrine Department of Pingyao County Hospital of Traditional Chinese Medicine,Pingyao,Shanxi,031100,China)

机构地区：[1]山西医科大学第九临床医学院,山西太原030000 [2]太原市中心医院内分泌科,山西太原030000 [3]平遥县中医院内分泌科,山西平遥031100

出　　处：《现代生物医学进展》2023年第24期4765-4770,共6页Progress in Modern Biomedicine

基　　金：山西省应用基础研究项目(201801D121361)。

摘　　要：目的:通过16S rRNA测序技术检测研究对象肠道菌群,探讨2型糖尿病足患者肠道菌群特征及其与主要临床生化指标的相关性,为基于肠道菌群途径治疗糖尿病足提供新的理论基础。方法:选取研究对象分为糖足组(A组,n=32)、糖尿病组(B组,n=32)、对照组(C组,n=31)三组,收集三组研究对象粪便标本及空腹血糖、糖化血红蛋白、炎症指标等主要临床生化指标,采用16S rRNA测序技术检测受试者肠道菌群,分析2型糖尿病足患者肠道菌群特征及其与主要临床化验指标的相关性。结果:1.与糖尿病组和对照组比较,糖足组血糖、血脂水平、炎性指标明显升高(P<0.05)。2.糖足组物种丰富度明显小于其他两组(P<0.05),三组间α多样性和β多样性存在差异(P<0.05)。3.糖足组中,弧菌属(尤以脱硫弧菌)、粪球菌属、厌氧棍状菌属等有害标志性物种丰度较其他两组增加,而双歧杆菌属、阿克曼菌属、普氏栖粪杆菌属、乳杆菌属等有益标志性菌种较其他两组减少。4.Spearman相关性分析表明瘤胃球菌属与FPG、HbA1c、WBC、NE%、CRP、ESR等指标成负相关(P<0.05),志贺菌属与WBC、NE%、CRP、ESR等指标成正相关(P<0.05),脱硫弧菌属与FPG、HbA1c、TG、WBC、NE%、CRP等指标成正相关(P<0.05)。结论:糖足组肠道菌群更加紊乱,而紊乱的肠道菌群特征与糖足组更加紊乱的糖代谢、脂质代谢以及更严重的炎症状态有关。因此,肠道菌群紊乱可能与2型糖尿病足的发生发展密切相关。Objective:16S rRNA sequencing technology was used to detect the intestinal flora of the research subjects,explore the intestinal flora characteristics and its correlation with Clinical biochemical index in patients wihe type 2 diabetic foot,and provide a new theoretical basis for the treatment of diabetic foot based on intestinal flora.Methods:The research objects were divided into three groups:diabetic foot group(A,n=32),diabetic group(B,n=32),and control group(C,n=31),and collected fecal samples and clinical biochemical indexes such as fasting blood glucose,HbA1c and inflammatory indexes.16S rRNA sequencing technology was used to detect the intestinal flora of the objects and analyze the intestinal flora characteristics of patients with type 2 diabetic foot and its correlation with clinical biochemical indexes.Results:1.Compared with the diabetic group and control group,blood glucose,lipid levels and inflammatory indexes were significantly increased in the diabetic foot group(P<0.05).2.The species richness of the diabetic foot group was significantly lower than that of the other two groups(P<0.05),and there were differences in alpha diversity andβdiversity among the three groups(P<0.05).3.In the diabetic foot group,the abundance of harmful iconic species such as Vibrio(especially desulfovibrio),Coprococcus and anaerobic Claviculus increased compared with the other two groups,while the beneficial iconic species such as bifidobacterium,Akkermania,Coprobiotic and Lactobacillus decreased compared with the other two groups.5.Spearman correlation analysis showed that Ruminococcus was negatively correlated with FPG,HbA1c,WBC,NE%,CRP and ESR(P<0.05),while Shigella was positively correlated with WBC,NE%,CRP and ESR(P<0.05).Desulfovibrio was positively correlated with FPG,HbA1c,TG,WBC,NE%and CRP(P<0.05).Conclusion:The intestinal flora in the diabetic foot group was more disordered,and the characteristics of the disordered intestinal flora were associated with more disordered glucose metabolism,lipid metabolism and more

关 键 词：2型糖尿病足 肠道菌群 肠道菌群紊乱 16S rRNA测序 

分 类 号：R587.2[医药卫生—内分泌]