痰液中细菌生物被膜镜检在慢性支气管扩张治疗中的价值分析  

Value analysis of the examination of bacterial biofilms in the treatment of chronic bronchiectasis

作　　者：李继刚 LI Jigang(Department of Laboratory,Tongshan District People's Hospital of Xuzhou City,Xuzhou,Jiangsu 221000,China)

机构地区：[1]徐州市铜山区人民医院检验科,江苏徐州221000

出　　处：《医药前沿》2024年第8期1-5,共5页Journal of Frontiers of Medicine

摘　　要：目的:分析细菌生物被膜(BF)镜检在慢性支气管扩张治疗中的价值。方法:选取2021年1月—2022年12月徐州市铜山区人民医院收治的240例慢性支气管扩张患者为研究对象,留取合格痰液标本,同时制备痰液涂片,革兰染色后检查BF,将BF阳性的标本进行细菌培养,根据培养和鉴定结果,挑选出铜绿假单胞菌阳性患者,并按照入院先后顺序,将其分为A组(未干预组,n=20)与B组(干预组,n=106)。统计BF阳性率,比较两组住院时间差异。结果:240例慢性支气管扩张患者的痰液中,检出BF 168例,BF检出率为70%。对168例BF阳性者进行痰液培养,培养出铜绿假单胞菌126例(75.00%),肺炎克雷伯菌22例(13.10%),鲍曼不动杆菌20例(11.90%)。B组住院时间短于A组,差异有统计学意义(P<0.05)。结论:慢性支气管扩张多与BF有关,尤其是铜绿假单胞菌BF;痰液细菌BF镜检对临床治疗有着重要指导意义。发现细菌BF后,及时报告,并对临床治疗进行指导性干预,能缩短患者住院时间。Objective Analysis of the value of bacterial biofilm(BF)microscopy in the treatment of chronic bronchiectasis.Methods 240 patients with chronic bronchiectasis admitted to Tongshan District People's Hospital in Xuzhou City from January 2021 to December 2022 were selected as the research subjects,and qualified sputum samples were collected.Gram stained smears were prepared and the microscopic was used to examine BF.The BF-positive samples were cultured for bacteria.Pseudomonas aeruginosa positive patients were selected and divided into two groups according to the order of admission:A(no intervention group,n=20)and B(intervention group,n=106).The positive rate of BF was calculated of the difference in hospitalization time between the two groups was compared.Results Among 240 patients with chronic bronchiectasis,168 cases of BF were detected in the sputum,with the positive rate of 70%.Sputum culture was conducted on 168 BF positive individuals,and 126 cases(75.00%)of Pseudomonas aeruginosa,22 cases(13.10%)of Klebsiella pneumoniae,and 20 cases(11.90%)of Acinetobacter baumannii were cultured.The hospitalization time of group B was shorter than that of group A,and the difference was statistically significant(P<0.05).Conclusions Especially pseudomonas aeruginosa BF,is a risk factor for bronchiectasis.The examination of BF is very important,and timely reporting of the discovery of BF and guiding intervention for clinical treatment can shorten the hospitalization stay of the patients.

关 键 词：慢性支气管扩张 生物被膜 镜检 治疗指导 

分 类 号：R562.2[医药卫生—呼吸系统]