慢性肾衰竭大鼠OAT1的表达对骨代谢的影响  

Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure

作　　者：沈文娟 梁纹鑫 李清 徐宏芳 张伟琴 李海欣 Wenjuan SHEN;Wenxin LIANG;Qing LI;Hongfang XU;weiqin ZHANG;Haixin LI(Pathology and Physiology Teaching and Research Office of Haiyuan College,Kunming Medical University;Dept.of Human Anatomy,,Kunming Medical University,Kunming Yunnan 651700;Medical Functionality Laboratory,Kunming Medical University,Kunming Yunnan 651700;Human Parasitology Teaching and Research Office,Kunming Medical University,Kunming Yunnan 651700;Dept.of Orthopaedics,Kunming 2nd People’s Hospital,Kunming Yunnan 650204,China)

机构地区：[1]昆明医科大学海源学院病理生理学教研室,昆明云南651700 [2]昆明医科大学海源学院人体解剖学教研室,昆明云南651700 [3]昆明医科大学海源学院医学机能学实验室,昆明云南651700 [4]昆明医科大学海源学院人体寄生虫学教研室,昆明云南651700 [5]昆明市第二人民医院骨科,昆明云南650204

出　　处：《昆明医科大学学报》2024年第2期32-38,共7页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(2021J0880,2024J1236)。

摘　　要：目的研究慢性肾衰竭时导致骨细胞有机阴离子转运蛋白(organic anion transporter,OAT)表达的变化,探讨OAT1表达对骨代谢的影响。方法将SD大鼠随机分为对照组(Control,n=6)和模型组(Model,n=6)。模型组采用“单肾切除+腺嘌呤灌胃法”建立大鼠慢性肾衰竭模型,通过血常规分析仪测定大鼠血清的红细胞(red blood cell,RBC)、血红蛋白(hemoglobin,Hb);通过全自动生化分析仪测定肌酐(creatinine,Cr)、尿素氮(urea nitrogen,BUN)、尿酸(uric acid,UA)、血钙(Ca^(2+))、血磷(P^(3+))等指标;对大鼠肾脏进行病理学检查;X线拍片检查大鼠胫骨标本;免疫组化检查骨组织OAT1表达。结果模型组大鼠的骨密度低于对照组;模型组大鼠钙磷代谢失调,并且骨组织结合OAT1值远低于对照组,差异具有统计学意义(P=0.0018)。结论慢性肾功衰竭影响OAT1在骨组织中的表达,导致钙磷代谢失调,从而加重肾性骨营养不良。Objective To investigate the changes of OAT expression in bone cells in chronic renal failure(CRF)and involved mechanism,and to explore the effect of OAT expression on bone metabolism.Methods Ra-ndomly divide the rats into a control group(n=6)and a model group(n=6).The model group established a rat chronic renal failure model using"single nephrectomy+adenine gavage"method,and the red blood cell(RBC)and hemoglobin(HGB)of the rat body were measured using a blood routine analyzer;Measure indicators such as creatinine(Cr),urea nitrogen(BUN),uricacid(UA),blood calcium(Ca^(2+)),and blood phosphorus(P^(3+))using a fully automated biochemical analyzer;Pathological examination of rat kidneys;X-ray examination of rat tibia;Immunohistochemical examination of bone tissue OAT1 level.Results The bone density of the model group rats is lower than that of the control group;The calcium and phosphorus metabolism of rats in the model group was in metabolic disorder,and the OAT1 value of bone tissue binding was much lower than that of the control group(P=0.0018),which was statistically significant.(P=0.0018)Conclusion Chronic renal failure affected the binding ability of OAT1 in bone tissue,leading to the metabolic disorder for calcium absorption and phosphorus metabolism,thus aggravating renal osteodystrophy(P<0.05).

关 键 词：有机阴离子转运蛋白 慢性肾功能衰竭 骨营养不良 

分 类 号：R956.1[医药卫生—药学]