遵义市食源性沙门菌分子分型及耐药研究  

Study on molecular typing and drug resistance of foodborne Salmonella in Zunyi City

作　　者：韦德琴 朱琳[1] WEI Deqin;ZHU Lin(Department of Laboratory,Zunyi Municipal Center for Disease Control and Prevention,Zunyi,Guizhou 563000,China)

机构地区：[1]贵州省遵义市疾病预防控制中心检验科,贵州遵义563000

出　　处：《检验医学与临床》2024年第6期789-792,799,共5页Laboratory Medicine and Clinic

基　　金：贵州省遵义市三代全基因组测序创新人才团队(遵市科人才[2022]9号)。

摘　　要：目的了解遵义市食源性沙门菌分型及耐药特征。方法收集遵义市2020-2022年食源性沙门菌,采用玻片凝集法对沙门菌进行血清分型,利用脉冲场凝胶电泳(PFGE)对沙门菌进行分子分型,采用肉汤稀释法对沙门菌进行耐药监测,应用BioNumerics软件进行数据分析。结果收集的21株沙门菌分为16种血清型,其中肯塔基沙门菌3株、德尔卑沙门菌2株、肠炎沙门菌2株、希林登沙门菌2株,其余均为1株。PFGE结果显示带型呈现多态性,同源性最高为62.96%。药敏试验结果显示食源性沙门菌对氨苄西林耐药率最高,为71.43%,其次对四环素耐药率达61.90%,多重耐药率为76.19%。结论遵义市食源性沙门菌血清型和遗传性多样,对抗菌药物的耐药率高,多重耐药严重。Objective To understand the typing and drug resistance characteristics of foodborne Salmonella in Zunyi City.Methods The foodborne Salmonella from 2020 to 2022 in Zunyi City were collected,and the slide agglutination method was used for serotyping of Salmonella,pulsed field gel electrophoresis(PFGE) was used for molecular typing of Salmonella,broth dilution method was used for drug resistance monitoring of Salmonella,and BioNumerics software was used for data analysis.Results The collected 21 strains of Salmonella were divided into 16 serotypes,including 3 strains of Salmonella Kentucky,2 strains of Salmonella Delphi,2 strains of Salmonella Enteritidis,2 strains of Salmonella Hillington and the rest were 1 strain;the PFGE results showed that the banding pattern was polymorphic with a highest homology of 62.96%;the drug sensitivity test results showed that the resistance rate of Salmonella from food sources to ampicillin was the highest(71.43%),followed by the resistance rate to tetracycline(61.90%),and the multiple drug resistance rate was 76.19%.Conclusion The Salmonella from food sources in Zunyi City present the serotype and genetic diversity with high resistant rate to antibiotics.The multi-drug resistance is serious.

关 键 词：食品 沙门菌 血清型 脉冲场凝胶电泳 耐药性 

分 类 号：R446.5[医药卫生—诊断学]