万古霉素和妥布霉素局部给药对大鼠下颌骨再生的影响  

Effect of local administration of vancomycin and tobramycin on mandibular regeneration in rats

作　　者：于凌佳 韩巍[2] 李星晨[3] 张蕾[4] YU Ling-jia;HAN Wei;LI Xing-chen(Department of Orthopaedics,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Traumatology,Beijing Jishuitan Hospital,Beijing 100035,China;Department of Orthopaedics,The First Affiliated Hospital of Zhengzhou University,Zhengzhou Henan 450052,China)

机构地区：[1]首都医科大学附属北京友谊医院骨科,北京100050 [2]北京积水潭医院创伤外科,北京100035 [3]郑州大学附属第一医院骨科,河南郑州450052 [4]中国医科大学附属口腔医院整形美容中心,辽宁沈阳110002

出　　处：《临床和实验医学杂志》2024年第3期229-233,共5页Journal of Clinical and Experimental Medicine

基　　金：辽宁省高等学校科学研究项目(编号:LJKQZ2021024)。

摘　　要：目的建立大鼠颌骨缺损模型,观察局部应用万古霉素和妥布霉素粉剂对体内骨再生的影响。方法按照随机数字表法将24只SD雄性大鼠分为4组,每组各6只。对照组无需给予抗生素,万古霉素组给予万古霉素88μg/g,妥布霉素组给予妥布霉素176μg/g,联合组给予万古霉素88μg/g和妥布霉素176μg/g。每只大鼠制作5 mm全厚度标准颌骨缺损模型,并分别在骨缺损处放置抗生素粉末。术后12周收集标本,通过微型计算机断层扫描(micro-CT)分析缺损区骨体积/总骨体积比(BV/TV)、骨形成面积(BFA)和骨体积(BV)。再通过苏木精和伊红(HE)染色和马松染色进行组织学评估,分析骨再生情况。结果全部实验动物均无死亡,无万古霉素或妥布霉素相关毒性症状出现,伤口均愈合良好,无手术并发症及伤口感染。从micro-CT扫描分析,万古霉素组缺损区BV/TV、BFA、BV分别为(8.59±2.23)%、(10.33±2.01)%、(1.73±0.35)mm^(3),均小于对照组[(21.67±3.51)%、(27.55±2.60)%、(3.53±0.35)mm^(3)],而妥布霉素组BV/TV、BFA、BV分别为(45.03±3.20)%、(48.88±4.07)%、(9.06±0.56)mm^(3),均大于对照组,联合组缺损区BV/TV、BFA、BV分别为(22.67±4.04)%、(27.64±3.44)%、(3.41±0.33)mm^(3),均大于万古霉素组,差异均有统计学意义(P<0.05);对照组与联合组比较,差异均无统计学意义(P>0.05)。HE染色和马松染色支持micro-CT的扫描结果。万古霉素组的大鼠的每视野区成骨细胞数、骨组织百分比分别为(1.67±1.21)个、(3.83±1.47)%,均小于对照组[(6.33±1.03)个、(11.67±2.16)%],而妥布霉素组大鼠的每视野区成骨细胞数、骨组织百分比分别为(11.17±1.47)个、(29.50±2.81)%,均大于对照组,联合组大鼠的每视野区成骨细胞数、骨组织百分比分别为(6.33±0.82)个、(9.83±2.32)%,均大于万古霉素组,差异均有统计学意义(P<0.05);对照组与联合组比较,差异均无统计学意义(P>0.05)。结论对于骨缺损,推荐局部应�Objective A mandibular defect rat model was established to investigate the osteogenic effect of local delivery vancomycin and tobramycin powder on bone regeneration.Methods A total of 24 SD male rats were divided into 4 groups according to the random number table method,with 6 rats in each group.The control group did not require antibiotics,while the vancomycin group received vancomycin 88 μg/g,tobramycin group administered tobramycin 176 μg/g,combined group administered with vancomycin 88 μg/g and tobramycin 176 μg/g.A 5 mm full-thickness standardized mandibular bone defect was performed with a drill in each rat and different antibiotic powders were placed over the bone defect space,respectively.All these animals were sacrificed after 12 weeks post-operation.The mandible bones were harvested for further radiographic and histologic analysis.The bone volume/total volume(BV/TV) ratio,bone volume(BV),and bone fractional area(BFA) in the defect area via micro-computed tomography(micro-CT scanning) were further analyzed.Then,the histology of rats was evaluated using hematoxylin and eosin(HE) staining and Masson staining,and the number of osteoblasts per filed was also analysed.Results There were no postoperative deaths,signs of vancomycin-related or tobramycin-related toxicity,or signs of systemic illness in any of the four groups.All wounds were healed well,and no complications or surgical site infection were observed in all rats.From the micro CT scanning analysis,the BV/TV,BFA,and BV in the defect area of the vancomycin group were(8.59±2.23)%,(10.33±2.01)%,and(1.73±0.35) mm^(3),respectively,which were smaller than those in the control group [21.67±3.51)%,(27.55±2.60)%,and(3.53±0.35) mm^(3)],while the BV/TV,BFA,and BV in the tobramycin group were(45.03±3.20)%,(48.88±4.07)%,and(9.06±0.56) mm^(3),respectively,which were larger than those in the control group,the BV/TV,BFA,and BV in the defect area of the combination group were(22.67±3.20)%,(27.64±3.44)%,and(3.41±0.33) mm^(3),which were higher than thos

关 键 词：大鼠 万古霉素 妥布霉素 骨生成 骨再生 局部给药 

分 类 号：R965[医药卫生—药理学]