泻白散质量标志物的发现及其作用机制预测分析  

Discovery of Q-markers of Xiebai Powder and Predictive Analysis of Its Mechanism of Action

作　　者：马天宇 肖智杰 段卓然 常梓睿 牟景龙 孟永海[1] 翟春梅[1] Ma Tianyu;Xiao Zhijie;Duan Zhuoran;Chang Zirui;Mou Jinglong;Meng Yonghai;Zhai Chunmei(Key Laboratory of Basic and Applied Research of Northen Medicine(Ministry of Education),Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,哈尔滨150040

出　　处：《中国药事》2024年第2期189-200,共12页Chinese Pharmaceutical Affairs

基　　金：哈尔滨市科技创新人才研究专项资金项目(编号2017RAQXJ125);黑龙江中医药大学研究生创新科研项目(编号2022yjscx055);黑龙江省博士后科研启动金资助项目(编号LBH-Q15137)。

摘　　要：目的:旨在明确泻白散质量标志物并预测其作用机制,确保临床疗效。方法:通过Q-markers“五原则”筛选泻白散质量标志物;采用TCMSP、Swiss Target Prediction等数据库获取Q-markers潜在作用靶点,构建质量标志物的蛋白互作网络。通过KEGG、GO、拓扑富集分析构建“关键靶点-关键通路-关键生物过程”网络,进而预测泻白散质量标志物的潜在作用机制。结果:研究显示,氧化白藜芦醇、绿原酸、阿魏酸、甘草素、甘草酸和芒柄花素6个化合物可作为泻白散的潜在质量标志物,这些物质可能通过调节炎症反应中的一氧化氮生物合成过程、AGEs-RAGE信号通路等途径发挥作用。结论:通过初步探讨泻白散的质量标志物及其作用机制,为泻白散质量控制与临床应用提供科学依据。Objective:To identify Q-markers and predict their mechanisms of action to ensure clinical effi cacy.Methods:The quality markers for Xiebai Powder were screened by Q-markers"Five Principles".Databases such as TCMSP and Swiss Target Prediction were used to obtain potential targets of Q-markers,in order to construct a protein interaction network for Q-markers.Through KEGG,GO,and topological enrichment analysis,the"key target-key signal pathway-key biological process"network was constructed to predict the potential mechanism of the Q-markers of Xiebai Powder.Results:This study showed that oxyresveratrol,chlorogenic acid,ferulic acid,liquiritigenin,glycyrrhizic acid and formononetin could be used as potential quality markers of Xiebai Powder.These substances might play a role in regulating the nitric oxide biosynthesis process and AGEs-RAGE signaling pathway in inflammatory response.Conclusion:This article preliminarily explores the quality markers and mechanism of Xiebai Powder,aiming to provide scientifi c basis for the quality control and clinical application of Xiebai Powder.

关 键 词：泻白散 五原则 质量标志物 作用机制 网络药理学 

分 类 号：R95[医药卫生—药学]