电针对急性心肌梗死大鼠心肌电重构的影响及其机制研究  

Effect of electroacupuncture on myocardial electrical remodeling in rats with acute myocardial infarction

作　　者：萨喆燕 潘晓华 朱小香 兰彩莲 万隆 罗来 许金森 SA Zhe-yan;PAN Xiao-hua;ZHU Xiao-xiang;LAN Cai-lian;WAN Long;LUO Lai;XU Jin-sen(Department of Meridian Research,Fujian Academy of Chinese Medical Sciences,Key Laboratory of Propagated Sensation Along Meridian of Fujian Province,Fuzhou 350003,China)

机构地区：[1]福建省中医药科学院经络研究所,福建省经络感传重点实验室,福州350003

出　　处：《针刺研究》2024年第2期155-163,共9页Acupuncture Research

基　　金：国家自然科学基金青年项目(No.81804001);福建省自然科学基金面上项目(No.2018J01858、2021J01912);福建省属公益类科研院所基本科研专项项目(No.2022R1003008)。

摘　　要：目的:探讨电针“内关”通过增强瞬时外向钾电流(Ito)改善急性心肌梗死大鼠心肌电重构的作用机制。方法:雄性SD大鼠随机分成对照组、模型组和电针组,每组10只。采用皮下注射异丙肾上腺素(85 mg/kg)诱导急性心肌梗死模型。电针组造模24 h后电针左侧“内关”,每次20 min,每日1次,连续5 d。TTC染色观察心肌坏死情况;HE染色观察心肌组织病理形态、测量心肌细胞横截面积;心电图记录ST段高度、QT间期(QT)、校正QT间期(QTc),计算QT间期离散率(QTd)和校正QT间期离散率(QTcd);RNA测序法检测心肌组织钾离子通道相关基因表达差异;实时荧光定量PCR法和Western blot法分别检测心肌组织Kchip2、Kv4.2的mRNA和蛋白表达。结果:TTC染色可见对照组心肌呈红色,模型组大鼠心肌大部分呈白色缺血样改变,电针组仅见心尖处心肌组织呈白色。与对照组比较,模型组大鼠心肌细胞横截面积显著增大(P<0.01),ST段显著抬高(P<0.01),QT、QTc、QTd和QTcd均显著增加(P<0.05,P<0.01);与模型组比较,电针组大鼠心肌细胞横截面积显著减小(P<0.01),ST段显著下降(P<0.01),QT、QTc、QTcd和QTd均显著减少(P<0.01,P<0.05)。RNA测序共筛选出20个3组共同表达的钾离子通道相关基因,电针组与模型组比较,其中Kchip2上调差异倍数最大。与对照组比较,模型组大鼠心肌组织Kchip2、Kv4.2 mRNA和蛋白表达均显著降低(P<0.01,P<0.05);与模型组比较,电针组大鼠心肌组织Kchip2、Kv4.2 mRNA和蛋白表达均显著升高(P<0.01,P<0.05)。结论:电针可改善心肌梗死大鼠心肌电重构,其机制可能与增加Ito主要成分Kchip2、Kv4.2表达有关。Objective To investigate the mechanism of electroacupuncture(EA)at“Neiguan”(PC6)in improving myocardial electrical remodeling in rats with acute myocardial infarction(AMI)by enhancing transient outward po⁃tassium current.Methods A total of 30 male SD rats were randomly divided into control,model and EA groups,with 10 rats in each group.The AMI model was established by subcutaneous injection with isoprenaline(ISO,85 mg/kg).EA was applied to left PC6 for 20 min,once daily for 5 days.Electrocardiogram(ECG)was recorded after treatment.TTC staining was used to observe myocardial necrosis.HE staining was used to observe the pathological morphology of myocardial tissue and measure the cross-sectional area of myocardium.Potassium ion-related genes in myocardial tis⁃sue were detected by RNA sequencing.The mRNA and protein expressions of Kchip2 and Kv4.2 in myocardial tissue were detected by real-time fluorescence quantitative PCR and Western blot,respectively.Results Compared with the control group,cardiomyocyte cross-sectional area in the model group was significantly increased(P<0.01),the ST segment was significantly elevated(P<0.01),and QT,QTc,QTd and QTcd were all significantly increased(P<0.05,P<0.01).After EA treatment,cardiomyocyte cross-sectional area was significantly decreased(P<0.01),the ST segment was significantly reduced(P<0.01),and the QT,QTc,QTcd and QTd were significantly decreased(P<0.01,P<0.05).RNA sequencing results showed that a total of 20 potassium ion-related genes co-expressed by the 3 groups were iden⁃tified.Among them,Kchip2 expression was up-regulated most notablely in the EA group.Compared with the control group,the mRNA and protein expressions of Kchip2 and Kv4.2 in the myocardial tissue of the model group were signifi⁃cantly decreased(P<0.01,P<0.05),while those were increased in the EA group(P<0.01,P<0.05).Conclusion EA may improve myocardial electrical remodeling in rats with myocardial infarction,which may be related to its functions in up-regulating the expressions of Kchip2 a

关 键 词：电针 心肌梗死 心肌电重构 瞬时外向钾电流 

分 类 号：R245[医药卫生—针灸推拿学]